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专题学术讲座
3月04日 INFECTIOUS DISEASES SEMINAR SERIES
发布时间:2013-02-27关键字:

  INFECTIOUS DISEASES SEMINAR SERIES

  

Dr. Xi Zhou

Principle Investigator

State Key Laboratory of Virology

College of Life Sciences

Wuhan University

 

Multiple Suppression of RNAi-mediated Antiviral Immunity by the Viral Suppressor of RNA Silencing (VSR) of Nodavirus inDrosophila Cells”

 

Time: 16:00, March 4 (Monday)

Place: Medical Science Building B321

Host: Ye Xiang

 

Abstract: RNA interference (RNAi) plays critical roles in cellular responses to viral infection in plants, insects and mammals. To escape from RNAi-mediated immunity, viruses encode viral suppressors of RNA silencing (VSRs) that sequester RNA duplexes and target protein components in the RNAi pathway. Although the endonuclease Dicers play critical roles in RNAi, the detailed mechanism by which VSRs antagonize Dicers remains unknown, and the interrelationship among diverse activities of VSRs evolved in RNA binding and interaction with protein components has not been investigated. Here, we show that from Wuhan Nodavirus (WhNV), B2 protein suppress Drosophila RNAi by the mechanism of interacting with Dicer-2 (Dcr-2) in addition to sequestering double-stranded RNA (dsRNA) and small interfering (siRNA). Our studies further reveal that B2 binds to the RNase III and PAZ domains of Dcr-2 through its C-terminal region, thereby blocking the activities of Dcr-2 in cleaving dsRNA into siRNA and incorporating siRNA into RISC. Moreover, we uncovered an interrelationship among diverse activities of WhNV B2, showing that RNA binding to B2 could enhance the B2-Dcr-2 interaction by promoting B2 dimerization. Altogether, our findings establish a model on multiple suppression of Drosophila RNAi by targeting both RNA and Dcr-2 with WhNV B2, and lead to a speculation that the synergistic effect among diverse activities of WhNV B2 might be a general mechanism of multi-functional VSRs

 

Light Refreshments will be served!!!

Background

The Centre for Infectious Diseases Research is holding a weekly seminar series. The seminars will be a great opportunity to hear speakers from both within and outside China speaking on a variety of subjects of interest to all those engaged in microbiology/immunology and allied disciplines. The coordinator of the seminar series is Babak Javid, and he would be delighted for fellow faculty to get in touch with regards to possible speakers to invite.

The seminars will also be of great interest to graduate students in life sciences, so we would appreciate PIs encouraging their students and post-docs to attend. By way of encouragement, we will be providing light refreshments to attendees.

If you are interested in meeting with the speaker, please email Babak Javid atbjavid@gmail.com.

We hope that this seminar series will prove to be a valuable addition for both training and scientific dialogue at Tsinghua, and look forward to seeing you there.

 

 




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