生命科学联合中心
学术报告
题 目: Fluorescent proteins: New forms and unexpected new functions.
报告人: Michael Z. Lin, M.D. / Ph.D.
Assistant Professor, Stanford Depts. of Pediatrics and Bioengineering
时 间: 2013年6月3日（星期一），下午 13:00-14:00 pm
地 点: 北京大学新生物楼邓佑才报告厅
主持人: 生命科学联合中心，李毓龙
相关下载：Michael Lin CV; 参考文献1；参考文献2；
Classical beta-barrel fluorescent proteins (FPs) are widely used as sensors of protein localization and activity, while other classes of light-absorbing proteins have been proposed for whole-body imaging in mammals and for optogenetic control of proteins with light. We will report on recent successes in engineering new forms of classical FPs that establish their utility for these applications as well. First, we performed structure-guided mutagenesis to engineer bright far-red FPs that provide substantially better brightness and signal over background in deep-tissue imaging compared to phytochrome-based proteins, enabling longitudinal visualization of stem cell differentiation in mice. Second, we discovered a previously unknown feature of variants of the photochromic green FP Dronpa, the ability to undergo light-dependent dissociation and association. To our knowledge, this is the first case of a domain acquiring light-responsive interaction ability via engineering in the laboratory. We further used this feature to create light-controllable proteins of a simple modular design that is generalizable and has self-reporting abilities, features that have yet to be achieved by other photoactive protein domains. Our findings extend the applications of FPs to new imaging modalities as well as beyond imaging to optogenetic control.
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