网站地图 加入收藏 中文 English
 
首页 中心概况 组织机构 研究队伍 科学研究 人才培养 交流合作 支撑服务 人才招聘 下载专区 联系我们
当前位置:首页 - 交流合作 - 学术会议及论坛
学术会议及论坛
4月3日 - 生物成像技术讲座通知
发布时间:2018-03-22关键字:

 

生物成像技术讲座通知

  生命学科校级平台及结构生物学高精尖创新中心邀请北京大学分子医学研究所陈良怡教授于43日下午2点在生物新馆143做关于高速超分辨成像讲座陈良怡教授现为北京大学分子医学研究所细胞分泌和代谢实验室主任,2012年获得国家自然科学基金委优秀青年基金项目支持,并成为Faculty of 1000组织的教授成员。陈教授的研究方向主要是超/高分辨率显微成像系统的研发及小型化的研究,并应用于研究糖尿病相关的钙信号和胰岛素分泌调控机制。

讲座时间201843  14:00-15:00

地点:生物新馆143

如要参加,请于42日下午17:00前知会马佳璐 (majialu@biomed.tsinghua.edu.cn)

邮件注明:姓名、院系、学号、联系方式、所在实验室

有兴趣与陈良怡教授面谈的老师请联系王亚林 (wyl2017@mail.tsinghua.edu.cn)

Ultrasensitive Hessian structured illumination microscopy enables ultrafast and long-term super-resolution imaging

Liangyi Chen

Institute of Molecular Medicine, Peking University

To increase the temporal resolution and maximal imaging time of super-resolution (SR) microscopy, we have developed a deconvolution algorithm for structured illumination microscopy based on Hessian matrixes (Hessian-SIM). It uses the continuity of biological structures in multiple dimensions as a priori knowledge to guide image reconstruction and attains artifact-minimized SR images with less than 10% of the photon dose used by conventional SIM while substantially outperforming current algorithms at low signal intensities. Hessian-SIM enables rapid imaging of moving vesicles or loops in the endoplasmic reticulum without motion artifacts and with a spatiotemporal resolution of 88 nm and 188 Hz. Its high sensitivity allows the use of sub-millisecond excitation pulses followed by dark recovery times to reduce photobleaching of fluorescent proteins, enabling hour-long time-lapse SR imaging of actin filaments in live cells. Finally, we observed the structural dynamics of mitochondrial cristae and structures that, to our knowledge, have not been observed previously, such as enlarged fusion pores during vesicle exocytosis.

 

 




版权所有 生命科学联合中心 京ICP备15006448号-5