Translational Fidelity and Mistranslation in the Cellular Response to Stress
Michael Ibba, PhD
Infectious Diseases Institute,
Department of Microbiology, Ohio State University
Abstract：Faithful translation of mRNA into the corresponding polypeptide is a complex multistep process, requiring accurate amino acid selection, transfer RNA (tRNA) charging and mRNA decoding on the ribosome. Key players in this process are aminoacyl-tRNA synthetases (aaRSs), which not only catalyze the attachment of cognate amino acids to their respective tRNAs, but also selectively hydrolyze incorrectly activated non-cognate amino acids and/or misaminoacylated tRNAs. This aaRS proofreading provides quality control checkpoints that exclude non-cognate amino acids during translation, and in so doing helps to prevent the formation of an aberrant proteome. However, despite the intrinsic need for high accuracy during translation, and the widespread evolutionary conservation of aaRS proofreading pathways, requirements for translation quality control vary depending on cellular physiology and changes in growth conditions, and translation errors are not always detrimental. Recent work has demonstrated that mistranslation can also be beneficial to cells, and some organisms have selected for a higher degree of mistranslation than others. Data will be presented to demonstrate how changes to protein translational fidelity impact mistranslation and lead to potentially beneficial responses to environmental and cellular stress.
References:
1. Mohler, K. and Ibba, M. (2017) Translational Fidelity and Mistranslation in the Cellular Response to Stress. Nature Microbiol. 2:17117.
Biography：
2017 - present Associate Director, Infectious Diseases Institute, Ohio State University, Columbus, Ohio
2013 - present Chair, Department of Microbiology, Ohio State University, Columbus, Ohio
2011 - present Co-Director, NIH Cellular, Molecular, and Biochemical sciences training program
2012 - 2013 Director, Biochemistry Graduate Program, Ohio State University, Columbus, Ohio
2010 - present Professor, Department of Microbiology, Ohio State University, Columbus, Ohio
2006 – 2010 Associate Professor, Department of Microbiology, Ohio State University, Columbus, Ohio
2001 – 2006 Assistant Professor, Department of Microbiology, Ohio State University, Columbus, Ohio
1999 – 2001 Associate Research Professor, Panum Institute, University of Copenhagen, Denmark
1995 – 1998 Associate Research Scientist, Department of Molecular Biophysics and Biochemistry, Yale University.
1993 – 1994 Postdoctoral Research Assistant, Microbiology Institute, Swiss Federal Institute of Technology, Switzerland.
1990 – 1993 Postdoctoral Research Fellow, Department of Biotechnology, Ciba-Geigy AG, Switzerland.
1990 Doctor of Philosophy, Department of Biochemistry and Applied Molecular Biology, University of Manchester, United Kingdom. Thesis: “The development of an anaerobic acetogenic continuous fermentation process”. Supervisor Dr. G.H. Fynn.
1986 Bachelor of Science, Imperial College, University of London, London, UK. Degree: Biochemistry, B.Sc.(Hons) 2(I), ARCS.
Time: April. 10th, 2018, 16:00
Venue: New Biology Building, Room 143
Host: Prof. Babak Javid
举办单位：生命科学联合中心
