传染病系列学术讲座：Application of bacterial type III secretion system in the reprogramming of eukaryotic cells
Speaker: Dr. Shouguang Jin
Professor,The College of Life Sciences,Nankai University
Time: 14:00, March 2nd (Friday)
Place: Medical Science Building B321,Tsinghua University
Host: Jingren Zhang
Abstract :
Bacterial type III secretion system is a surface apparatus designed to inject bacterial proteins directly into the cytosols of eukaryotic host cells, altering host cell signal transductions that facilitate the bacterial survival in the host environment. Utilizing this system, we were able to deliver nuclear proteins of our interest directly into both differentiated and pluripotent stem cells. Delivery of Cre recombinase triggered efficient loxP site mediated DNA recombination while delivery of MyoD, a key muscle regulatory factor, into fibroblasts triggered trans-differentiation into myocytes. Protein based technique provides a safe alternative to transgene expression mediated cellular reprogramming, amendable to clinical applications. These studies serve as a foundation for the bacterial delivery of transcription factors to efficiently modulate concentration-dependent and temporal activation of gene expression to direct cell fate without jeopardizing genomic integrity.
Light Refreshments will be served!
Background:
The Centre for Infectious Diseases Research is holding a weekly seminar series. The seminars will be a great opportunity to hear speakers from both within and outside China speaking on a variety of subjects of interest to all those engaged in microbiology/immunology and allied disciplines. The coordinator of the seminar series is Babak Javid, and he would be delighted for fellow faculty to get in touch with regards to possible speakers to invite.
The seminars will also be of great interest to graduate students in life sciences, so we would appreciate PIs encouraging their students and post-docs to attend. By way of encouragement, we will be providing light refreshments to attendees.
If you are interested in meeting with the speaker, please email Babak Javid at bjavid@gmail.com.
We hope that this seminar series will prove to be a valuable addition for both training and scientific dialogue at Tsinghua, and look forward to seeing you there.
We hope that this seminar series will prove to be a valuable addition for both training and scientific dialogue at Tsinghua, and look forward to seeing you there.
