钱伟强
电子邮件: wqqian(at)pku(dot)edu(dot)cn；
电话:
研究方向:
本实验室以拟南芥为模式系统，研究表观遗传学信息如何影响基因表达以及植物生长发育，主要研究方向为DNA去甲基化途径和转座子对附近基因的表达调控。DNA甲基化修饰是一种重要的表观遗传学标志，在基因的表达调控，基因组的结构及稳定性，X染色体的失活和其它生长发育调控等方面都具有十分重要的作用。DNA甲基化的水平主要由甲基化和去甲基化这两个途经来协同调控。在植物体内，DNA去甲基化主要是由repressor of silencing 1 (ROS1)基因家族介导的碱基切除修复机制来实现。在植物中对DNA甲基化途经的研究已经比较深入，但是对DNA去甲基化的分子机制还不清楚。我们将运用遗传学，生物化学和细胞生物学的方法，对植物体内DNA去甲基化的酶学机制以及调控去甲基化的途经进行深入的研究。主要研究方向包括：（1）鉴定参与DNA去甲基化的酶；（2）筛选并研究参与去甲基化调控的因子；（3）表观遗传学信息对基因的表达调控。
代表性科研论文:
1. Qian W*, Miki D*, Zhang H, Liu Y, Zhang X, Tang K, Kan Y, La H, Li X, Li S, Zhu X, Shi X, Zhang K, Pontes O, Chen X, Liu R, Gong Z and Zhu JK. A Histone Acetyltransferase Regulates Active DNA Demethylation in Arabidopsis. Science. 2012 Jun 15;336(6087):1445-8.
2. Li X, Qian W, Zhao Y, Wang C, Shen J, Zhu J-K and Gong Z. The anti-Silencing role of the RNA-directed DNA methylation pathway and a histone acetyltransferase in Arabidopsis. Proc Natl Acad Sci U S A. 2012 Jul 10;109(28):11425-30.
3. Martínez-Macías M*, Qian W*, Miki D*, Pontes O, Liu Y, Tang K, Liu R, Morales-Ruiz T, R. Ariza R, Roldán-Arjona R, and Zhu JK. A DNA 3’-phosphatase functions in active DNA demethylation in Arabidopsis. Mol Cell. 2012 Feb10; 45(3): 357-70. (*Contributed equally to this work)
4. Mang HG*, Qian W*, Zhu Y, Qian J, Kang HG, F.Klessig D and Hua J. ABA deficiency antagonizes high temperature inhibition of disease resistance through enhancing nuclear accumulation of resistance proteins SNC1 and RPS4 in Arabidopsis. Plant Cell. 2012 Mar; 24 (3): 1271-84. (*Contributed equally to this work)
5. Wang L*, Li Z*, Qian W, Guo W, Gao X, Huang L, Wang H, Zhu H, Wu JW, Wang D and Liu D. Arabidopsis purple acid phosphatase AtPAP10 is predominantly associated with the root surface and play an important role in plant tolerance to phosphate limitation. Plant Physiol. 2011 Nov; 157 (3): 1283-99.
6. Tran HT, Qian W, Hurley BA, She YM, Wang D, and Plaxton WC. Biochemical and molecular characterization of AtPAP12 and AtPAP26: the predominant purple acid phosphatase isozymes secreted by phosphate-starved Arabidopsis thaliana. Plant Cell Environ. 2010 Nov;33 (11): 1789-803
7. Gao Z, Liu HL, Daxinger L, Pontes O, He X, Qian W, Lin H, Xie M, Lorkovic ZJ, Zhang S, Miki D, Zhan X, Pontier D, Lagrange T, Jin H, Matzke AJ, Matzke M, Pikaard CS and Zhu JK. An RNA polymerase II- and AGO4-associated protein acts in RNA-directed DNA methylation. Nature. 2010 May 6;465(7294):106-9.
8. Zhu Y, Qian W, and Hua J. Temperature modulates plant defense responses through NB-LRR proteins. PLoS Pathog. 2010 Apr 1;6 (4):e1000844