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魏文胜

    

魏文胜

 

电子邮件:wswei (at)pku(dot)edu(dot)cn

研究领域:基因组编辑、疾病与感染

 

办公室:北京市海淀区颐和园路5号北京大学生命科学学院201室 100871 

  

研究方向: 

着重发展真核基因修饰技术(i.e. TALE & CRISPR system)及高通量功能基因组学,在此基础上通过多种手段研究人源宿主细胞参与病原微生物感染的蛋白、信号通路及分子机制,为发展宿主导向的治疗手段提供新的药物靶点和思路。在生物技术方面关注真核基因定点修饰、功能基因组学以及基因治疗;对疾病与感染的机制研究,特别关注艰难梭菌(Clostridium difficile)毒素侵染机制以及病毒性肝炎(i.e. HCV)的致病机理和治疗。

 

代表性科研论文: 

1. He R, Peng J, Yuan P, Xu F, Wei W*. (2015) Divergent roles of BECN1 in LC3 lipidation and autophagosomal function. Autophagy (in press).
2. Peng J, Zhou Y, Zhu S and Wei W*. (2015) High-throughput Screens in Mammalian Cells Using the CRISPR-Cas9 System. FEBS Journal, DOI: 10.1111/febs.13251.
3. Ren Q, Li C, Yuan P, Cai C, Luo G, Zhang L and Wei W*. (2015) A Dual-Reporter System for Real-Time Monitoring and High-throughput CRISPR/Cas9 Library Screening of the Hepatitis C Virus. Sci. Rep. 5, 8865; DOI:10.1038/srep08865.
4. Shen J, Cai C, Yu Z, Pang Y, Zhou Y, Qian L, Wei W* and Huang Y*. (2015) A microfluidic live cell assay to study anthrax toxin induced cell lethality assisted by conditioned medium. Sci. Rep. 5, 8651; DOI:10.1038/srep08651.
5. Yuan P, Zhang H, Cai C, Zhu S, Zhou Y, Yang X, He R, Li C, Guo S, Li S, Huang T, Feng H and Wei W*. (2015) Chondroitin sulfate proteoglycan 4 functions as the cellular receptor for Clostridium difficile toxin B. Cell Res. 25:157-168; published online 30 December 2014. (Cover story)
6. Zhou Y, Zhu S, Cai C, Yuan P, Li C, Huang Y and Wei W*. (2014) High-throughput Screening of a CRISPR/Cas Library for Functional Genomics in Human Cells. Nature 509(7501): 487-91.
7. Qian LL, Cai CZ, Yuan PF, Jeong SY, Yang XZ, Almeida VD, Ernst J, Costa M, Cohen SN, Wei WS*. Bidirectional effect of Wnt signaling antagonist DKK1 on the modulation of anthrax toxin uptake. Sci China Life Sci, 2014, 57: 469–481. (Cover story)
8. Yang J, Zhang Y, Yuan P, Cai C, Ren Q, Guo S, Zhu C, Qi H and Wei W*. (2014) A Complete Decoding of DNA Recognition by TAL Effector RVDs. Cell Res. 24:628-631.
9. Yang J, Yuan P, Wen D , Sheng Y, Zhu S, Yu Y, Gao X and Wei W*. (2013) ULtiMATE system for rapid assembly of customized TAL effectors. PLoS One 2013; 8:e75649.
10. Wei W, Lu Q., Chaudry J, Leppla S, Cohen SN*. (2006) The LDL receptor-related protein LRP6 mediates internalization and lethality of anthrax toxin. Cell 124, 1141-1154.
11. Wei W, PlovanichJones A, Deng W, Jin Q, Collmer A, Huang H, and He SY*. (2000) The gene coding for the Hrp pilus structural protein is required for type III secretion of Hrp and Avr proteins in Pseudomonas syringae pv. tomato. Proc. Natl. Acad. Sci. USA 97, 2247-52.
12. Roine E, Wei W^, Yuan J, Nurmiaho-Lassila EL, Kalkkinen N, Romantschuk M, He SY*. (1997) Hrp pilus: an hrp-dependent bacterial surface appendage produced by Pseudomonas syringae pv. tomato DC3000. Proc. Natl. Acad. Sci. USA 94, 3459-64. (^Co-first author)

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