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汤富酬

汤富酬

 

电子邮件:tangfuchou (at)pku(dot)edu(dot)cn

研究领域:干细胞、表观遗传学

 

办公室:北京市海淀区颐和园路5号 100871 

  

研究方向: 

主要研究方向为人类早期胚胎发育过程中基因表达网络的表观遗传学调控。系统发展了单细胞功能基因组学研究体系,建立了单细胞RNA-Seq转录组高通量测序技术、单细胞DNA甲基化组高通量测序技术,并利用这一技术体系对人类早期胚胎进行了深入、系统的分析,发现了人类早期胚胎中基因表达网络的重要表观遗传学调控机理。在国际上首次对人类卵细胞进行了单细胞高通量基因组测序,阐明了人类卵细胞减数分裂的关键生物学特征,实现了利用单细胞高通量基因组测序技术进行植入前遗传学诊断、以大幅度提高试管婴儿成功率。在此基础上阐明了人类早期胚胎发育以及胚胎干细胞建系过程中的基因表达网络的动态变化,发现在早期胚胎以及胚胎干细胞建系过程中多能性细胞内可变剪接、细胞代谢等方面的模式转变。在国际上首次实现了对人类早期胚胎发育过程中DNA甲基化组的系统研究,揭示了人类早期胚胎DNA去甲基化过程的异质性以及其他关键特征,为人们提供了一个全面分析人类早期胚胎DNA甲基化调控网络的研究框架。在国际上首次发现小鼠早期胚胎中母源和父源基因组在单细胞的受精卵阶段均会发生大规模的DNA主动和被动去甲基化,并且DNA双加氧酶Tet3介导了主动去甲基化的发生,而糖苷酶TDG并不参与这一过程,暗示在Tet3介导的甲基化胞嘧啶氧化途径的下游存在着其它蛋白负责完成甲基化胞嘧啶氧化产物的清除,实现DNA的主动去甲基化。

 

代表性科研论文: 


1. Zhou F, Li X, Wang W, Zhu P, Zhou J, He W, Ding M, Xiong F, Zheng X, Li Z, Ni Y, Mu X, Wen L, Cheng T, Lan Y, Yuan W*, Tang Fuchou*, Liu B*. Tracing haematopoietic stem cell formation at single-cell resolution. Nature, 2016, doi: 10.1038/nature17997 (*: Co-corresponding authors)
2. Wen L*, Tang Fuchou*. Single-cell sequencing in stem cell biology. Genome Biology, 2016, 17: 71 (Review) (*: Co-corresponding authors).
3. Hou Y, Guo H, Cao C, Li X, Hu B, Zhu P, Wu X, Wen L, Tang Fuchou*, Huang Y*, Peng J*. Single-cell triple omics sequencing reveals genetic, epigenetic, and transcriptomic heterogeneity in hepatocellular carcinomas. Cell Research, 2016, 26: 304-319 (*: Co-corresponding authors).
4. Pan H, Guan D, Liu X, Li J, Wang L, Wu J, Zhou J, Zhang W, Ren R, Zhang W, Li Y, Yang J, Hao Y, Yuan T, Yuan G, Wang H, Ju Z, Mao Z, Li J, Qu J*, Tang Fuchou*, Liu GH*. SIRT6 safeguards human mesenchymal stem cells from oxidative stress by coactivating NRF2. Cell Research, 2016, 26: 190-205 (*: Co-corresponding authors).
5. Yan L, Guo H, Hu B, Li R, Yong J, Zhao Y, Zhi X, Fan X, Guo F, Wang X, Wang W, Wei Y, Wang Y, Wen L, Qiao J*, Tang Fuchou*. Epigenomic landscape of human fetal brain, heart, and liver. Journal of Biological Chemistry, 2016, 291: 4386-4398 (*: Co-corresponding authors).
6. Wen L*, Tang Fuchou*, How to catch rare cell types. Nature, 2015, 525: 197-198 (Preview) (*: Co-corresponding authors).
7. Guo F, Yan L, Guo H, Li L, Hu B, Zhao Y, Yong J, Hu Y, Wang X, Wei Y, Wang W, Li R, Yan J, Zhi X, Zhang Y, Jin H, Zhang W, Hou Y, Zhu P, Li J, Zhang L, Liu S, Ren Y, Zhu X, Wen L, Gao YQ, Tang Fuchou*, Qiao J*. The transcriptome and DNA methylome landscapes of human primordial germ cells. Cell, 2015, 161: 1437-1452 (*: Co-corresponding authors).
8. Zhang W, Li J, Suzuki K, Qu J, Wang P, Zhou J, Liu X, Ren R, Xu X, Ocampo A, Yuan T, Yang J, Li Y, Shi L, Guan D, Pan H, Duan S, Ding Z, Li M, Yi F, Bai R, Wang Y, Chen C, Yang F, Li X, Wang Z, Aizawa E, Goebl A, Soligalla RD, Reddy P, Esteban CR, Tang Fuchou*, Liu GH*, Belmonte JC*. A Werner syndrome stem cell model unveils heterochromatin alterations as a driver of human aging. Science, 2015, 348: 1160-1163 (*: Co-corresponding authors).
9. Wen L*, Tang Fuchou*, Charting a Map through the Cellular Reprogramming Landscape. Cell Stem Cell, 2015, 16: 215-216 (Preview) (*: Co-corresponding authors).
10. Wen L, Li J, Guo H, Liu X, Zheng S, Zhang D, Zhu W, Qu J, Guo L, Du D, Jin X, Zhang Y, Gao Y, Shen J, Ge H, Tang Fuchou*, Huang Y*, Peng J*. Genome-scale detection of hypermethylated CpG islands in circulating cell-free DNA of hepatocellular carcinoma patients. Cell Research, 2015, 25: 1250-1264 (*: Co-corresponding authors).
11. Yan L, Huang L, Xu L, Huang J, Ma F, Zhu X, Tang Y, Liu M, Lian Y, Liu P, Li R, Lu S, Tang Fuchou*, Qiao J*, Xie XS*. Live births after simultaneous avoidance of monogenic diseases and chromosome abnormality by next-generation sequencing with linkage analyses. Proc Natl Acad Sci U S A, 2015, 112: 15964-15969 (*: Co-corresponding authors).
12. Fan X, Zhang X, Wu X, Guo H, Hu Y, Tang Fuchou*, Huang Y*. Single-cell RNA-seq transcriptome analysis of linear and circular RNAs in mouse preimplantation embryos. Genome Biology, 2015, 16: 148 (*: Co-corresponding authors)
13. Guo H, Zhu P, Guo F, Li X, Wu X, Fan X, Wen L*, Tang Fuchou*. Profiling DNA methylome landscapes of mammalian cells with single-cell reduced-representation bisulfite sequencing. Nature Protocols, 2015, 10: 645-659 (*: Co-corresponding authors).
14. Duan S, Yuan G, Liu X, Ren R, Li J, Zhang W, Wu J, Xu X, Fu L, Li Y, Yang J, Zhang W, Bai R, Yi F, Suzuki K, Gao H, Esteban CR, Zhang C, Izpisua Belmonte JC, Chen Z, Wang X, Jiang T, Qu J*, Tang Fuchou*, Liu GH*. PTEN deficiency reprogrammes human neural stem cells towards a glioblastoma stem cell-like phenotype. Nature Communications, 2015, 6: 10068 (*: Co-corresponding authors).
15. Wu Y, Zhou H, Fan X, Zhang Y, Zhang M, Wang Y, Xie Z, Bai M, Yin Q, Liang D, Tang W, Liao J, Zhou C, Liu W, Zhu P, Guo H, Pan H, Wu C, Shi H, Wu L* , Tang Fuchou*, Li J,*. Correction of a Genetic Disease by CRISPR-Cas9-Mediated Gene Editing in Mouse Spermatogonial Stem Cells. Cell Research, 2015 , 25: 67-79 (*: Co-corresponding authors).
16. Shen J, Jiang D, Fu Y, Wu X, Guo H, Feng B, Pang Y, Streets AM, Tang Fuchou*, Huang Y*. H3K4me3 epigenomic landscape derived from ChIP-Seq of 1 000 mouse early embryonic cells. Cell Research, 2015, 25: 143-147 (*: Co-corresponding authors).
17. Guo F, Li X, Liang D, Li T, Zhu P, Guo H, Wu X, Wen L, Gu TP, Hu B, Walsh CP, Li J*, Tang Fuchou*, Xu GL*. Active and Passive Demethylation of Male and Female Pronuclear DNA in the Mammalian Zygote. Cell Stem Cell, 2014, 15: 447-458 (*: Co-corresponding authors).
18. Guo H, Zhu P, Yan L, Li R, Hu B, Lian Y, Yan J, Ren X, Lin S, Li J, Jin X, Shi X, Liu P, Wang X, Wang W, Wei Y, Li X, Guo F, Wu X, Fan X, Yong J, Wen L, Xie SX, Tang Fuchou*, Qiao J*. The DNA methylation landscape of human early embryos. Nature, 2014, 511: 606-610 (*: Co-corresponding authors).
19. Wen L, Tang Fuchou*, Reconstructing Complex Tissues from Single-Cell Analyses. Cell, 2014, 157: 771-773 (*: Corresponding author).
20. Wen L, Li X, Yan L, Tan Y, Li R, Zhao Y, Wang Y, Xie J, Zhang Y, Song C, Yu M, Liu X, Zhu P, Li X, Hou Y, Guo H, Wu X, He C*, Li R*, Tang Fuchou*, Qiao J*. Whole-genome analysis of 5-hydroxymethylcytosine and 5-methylcytosine at base resolution in the human brain. Genome Biology, 2014, 15: R49 (*: Co-corresponding authors).
21. Streets AM, Zhang X, Cao C, Pang Y, Wu X, Xiong L, Yang L, Fu Y, Zhao L*, Tang Fuchou*, Huang Y*. Microfluidic single-cell whole-transcriptome sequencing. Proc Natl Acad Sci U S A, 2014, 111: 7048-7053 (*: Co-corresponding authors).
22. Hou Y, Fan W, Yan L, Li R, Lian Y, Huang J, Li J, Xu L, Tang Fuchou*, Xie XS*, Qiao J*. Genome Analyses of Single Human Oocytes. Cell, 2013, 155: 1492-1506 (*: Co-corresponding authors).
23. Guo H, Zhu P, Wu X, Li X, Wen L, Tang Fuchou*. Single-cell methylome landscapes of mouse embryonic stem cells and early embryos analyzed using reduced representation bisulfite sequencing. Genome Research, 2013, 23: 2126-2135 (*: Corresponding author).
24. Yan L, Yang M, Guo H, Yang L, Wu J, Li R, Liu P, Lian Y, Zheng X, Yan J, Huang J, Li M, Wu X, Wen L, Lao K, Li R*, Qiao J*, Tang Fuchou*. Single-cell RNA-Seq profiling of human preimplantation embryos and embryonic stem cells. Nature Structural & Molecular Biology, 2013, 20: 1131-1139 (*: Co-corresponding authors).
25. Gan H, Wen L, Liao S, Lin X, Ma T, Liu J, Song CX, Wang M, He C, Han C*, Tang Fuchou*. Dynamics of 5-hydroxymethylcytosine during mouse spermatogenesis. Nature Communications, 2013, 4: 1995 (*: Co-corresponding authors).
26. Tang F*. Small RNAs in mammalian germline: tiny for immortal. Differentiation, 2010, 79: 141-146 (Review) (*: Corresponding author)


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