姓名：李赛

职称：副教授

 

联系方式：

邮箱：sai (at) tsinghua.edu.cn

通讯地址：清华大学生物医学馆

实验室网站：http://www.lisailab.com

 

学习及工作经历：

2018年-至今：  清华大学生命学院    研究员

2018年-至今：  清华-北京大学生命联合中心    研究员      

2018年-至今：  结构生物学高精尖创新中心    研究员              

2017－2018年：  英国牛津大学粒子成像中心    Senior Research Associate                     

2013－2017年：  英国牛津大学粒子成像中心    博士后           

2009－2012年：  德国哥廷根大学生物物理系    博士        

2006－2008年：  德国斯图加特大学物理系    硕士

2002－2006年：  武汉大学应用物理系    学士

研究方向：

我们研究：1）高分辨冷冻电镜断层成像（cryo-Electron Tomography, cryo-ET）方法开发；2）新发致病型囊膜病毒的组装及通过膜融合去组装的结构与功能。

高分辨cryo-ET是通过收集三维断层图像，再使用子断层图像平均法（sub-tomogram averaging, STA）进行结构解析的跨尺度结构生物学方法。由于cryo-ET数据包含精确的Z轴信息，它对样品纯度及构象均一性要求较低，且在制备样品过程中无需使其脱离原生环境，获得的结构更具生理意义。

包括新冠病毒在内的囊膜病毒是对现代人类健康最具威胁的病原体。由于形态不均一，囊膜病毒的原位结构一直是个挑战。我们使用cryo-ET解析全病毒结构，及在细胞中直接观测病毒生命活动的原位结构，希望回答以下重要问题：囊膜病毒如何组装，又如何通过膜融合去组装？可否找到病毒在这个过程中的弱点？

代表性论文:

1. Sai Li: Cryo-electron tomography of enveloped viruses. Trends in Biochemical Sciences, 2021 (In press)

2. Joel D. Allen, Himanshi Chawla, Firdaus Samsudin, Lorena Zuzic, Aishwary Tukaram Shivgan, Yasunori Watanabe, Wan-ting He, Sean Callaghan, Ge Song, Peter Yong, Philip J. M. Brouwer, Yutong Song, Yongfei Cai, Helen M. E. Duyvesteyn, Tomas Malinauskas, Joeri Kint, Paco Pino, Maria J. Wurm, Martin Frank, Bing Chen, David I. Stuart, Rogier W. Sanders, Raiees Andrabi, Dennis R. Burton, Sai Li, Peter J. Bond, and Max Crispin: Site-Specific Steric Control of SARS-CoV 2 Spike Glycosylation. Biochemistry, 07/2021; DOI: 10.1021/acs.biochem.1c00279

3. Hangping Yao*, Yutong Song*, Yong Chen*, Nanping Wu*, Jialu Xu*, Chujie Sun, Jiaxing Zhang, Tianhao Weng, Zheyuan Zhang, Zhigang Wu, Linfang Cheng, Danrong Shi, Xiangyun Lu, Jianlin Lei, Max Crispin, Yigong Shi, Lanjuan Li#, and Sai Li#: Molecular architecture of the SARS-CoV-2 virus. Cell, 2020; DOI:10.1016/J.CELL.2020.09.018

4. Alexandra Serris, Robert Stass, Eduardo A. Bignon, Nicolas A. Muena, Jean-Claude Manuguerra, Rohit K. Jangra, Sai Li, Kartik Chandran, Nicole Tischler, Juha T. Huiskonen, Felix A. Rey, and Pablo Guardado-Calvo: Description of the Hantavirus Surface Glycoprotein Lattice and Its Membrane Fusion Control Mechanism. Cell, 2020; DOI:10.1016/J.CELL.2020.08.023

5. Ying Liu*, Yutong Song*, Siyu Zhang, Min Diao, Shanjin Huang, Sai Li, and Xu Tan: PSGL-1 inhibits HIV-1 infection by restricting actin dynamics and sequestering HIV envelope proteins. Cell Discovery, 12/2020; DOI:10.1038/s41421-020-0184-9

6. Yanqing Zhang*, Sai Li*, Xuechen Zhu*, Gaoxingyu Huang*, Chao Zeng*, Qifan Wang, Kunpeng Wang, Qiang Zhou, Wusheng Zhang, Guangwen Yang, Minhao Liu, Qinghua Tao, Jianlin Lei, and Yigong Shi: Molecular architecture of the luminal ring of the Xenopus laevis nuclear pore complex. Cell Research, 05/2020; DOI:10.1038/s41422-020-0320-y

7. Xiaoling Wu*, Hua Yue*, Yuanyu Zhang*, Xiaoyong Gao, Xiaoyang Li, Licheng Wang, Yufei Cao, Miao Hou, Haixia An, Lin Zhang#, Sai Li#, Jingyuan Ma, He Lin, Yanan Fu, Hongkai Gu, Wenyong Lou, Wei Wei#, Richard N. Zare, and Jun Ge#: Packaging and delivering enzymes by amorphous metal-organic frameworks. Nature Communications, 11/2019; 10(1)., DOI:10.1038/s41467-019-13153-x

8. Kamel El Omari, Sai Li, Abhay Kotecha, Thomas S Walter, Eduardo Andres Bignon, Karl Harlos, Pentti Somerharju, Felix De Haas, Daniel K. Clare, Mika Molin, Felipe Hurtado, Mengqiu Li, Jonathan M. Grimes, Dennis H. Bamford, Nicole D. Tischler, Juha T. Huiskonen, David I. Stuart, Elina Roine: The structure of a prokaryotic viral envelope protein expands the landscape of membrane fusion proteins. Nature Communications, 02/2019; 10(1)., DOI:10.1038/s41467-019-08728-7

9. Steinar Halldorsson*, Sai Li*, Mengqiu Li, Karl Harlos, Thomas A. Bowden, Juha T. Huiskonen: Shielding and activation of a viral membrane fusion protein. Nature Communications, 01/2018; 9(349)., DOI:10.1038/s41467-017-02789-2

10. Sai Li, Ilona Rissanen, Antra Zeltina, Jussi Hepojoki, Jayna Raghwani, Karl Harlos, Oliver G. Pybus, Juha T. Huiskonen, Thomas A. Bowden: A Molecular-Level Account of the Antigenic Hantaviral Surface. Cell Reports, 06/2016; 16(1):278., DOI:10.1016/j.celrep.2016.06.039

11. Sai Li, Zhaoyang Sun, Rhys Pryce, Marie-Laure Parsy, Sarah K Fehling, Katrin Schlie, C Alistair Siebert, Wolfgang Garten, Thomas A Bowden, Thomas Strecker, Juha T Huiskonen: Acidic pH-Induced Conformations and LAMP1 Binding of the Lassa Virus Glycoprotein Spike. PLoS Pathogens, 02/2016; 12(2)., DOI:10.1371/journal.ppat.1005418

12. Sai Li*, Christian Sieben*, Kai Ludwig, Chris T Höfer, Salvatore Chiantia, Andreas Herrmann, Frederic Eghiaian, Iwan A.T. Schaap: pH-Controlled Two-Step Uncoating of Influenza Virus. Biophysical Journal, 04/2014; 106(7):1447-56., DOI:10.1016/j.bpj.2014.02.018

13. Sai Li, Frederic Eghiaian, Christian Sieben, Andreas Herrmann, Iwan A.T. Schaap: Bending and Puncturing the Influenza Lipid Envelope. Biophysical Journal, 02/2011; 100(3):637-45., DOI:10.1016/j.bpj.2010.12.3701

14. Felix Hoos, Sai Li, Todd Meyrath, Bernd Braun, and Harald Giessen: Thermal lensing in an end-pumped Yb:KGW slab laser with high power single emitter diodes. Optics Express, 04/2008; 16(9):6041-9., DOI:10.1364/OE.16.006041