陈雪梅

 

电子邮箱： xuemei.chen(at)pku(dot)edu(dot)cn

 

研究方向：

本课题组主要研究基因表达转录后调控在植物生长发育，环境响应等生物过程中的作用。目前的主要研究方向之一为microRNA和 siRNA代谢与翻译的关系, microRNA的细胞间运输机制及作为信号分子调控植物响应温度变化的机制。第二个主要研究方向为mRNA的非典型加帽修饰， 比如NAD， FAD， dpCoA 帽子的加入，去除机制及分子功能。已经开发或正在开发利用LC-MS 及组学方法鉴定非典型加帽。第三个刚起步的研究方向为叶绿体的发育， 基因表达， 及RNA 修饰。

 

 

代表性论文：

Selected papers on small RNAs

(* corresponding authorship)

1.      Qing Sang, Lusheng Fan, Tianxiang Liu, YongjianQiu, Juan Du, Beixin Mo, Meng Chen*, and Xuemei Chen*. (2023). MicroRNA156 conditions auxin sensitivity to enable growth plasticity in response to environmental changes in Arabidopsis. Nature Comm., accepted.

2.     Yuan Wang, Brandon H. Le, Jianqiang Wang, Chenjiang You, Yonghui Zhao, Mary Galli, Ye Xu, Andrea Gallavotti, Thomas Eulgem, Beixin Mo*, and Xuemei Chen*. (2022). A novel factor that recruits and excludes Pol IV-mediated DNA methylation in a site-specific manner. Science Advances 8, DOI: 10.1126/sciadv.adc9454.

3.     Lusheng Fan, Cui Zhang, Yong Zhang, Ethan Stewart, Jakub Jez, Keiji Nakajima, and Xuemei Chen*. (2022). Microtubules promote the non-cell autonomous action of microRNAs by inhibiting their cytoplasmic loading onto ARGONAUTE1 in Arabidopsis. Dev. Cell. 57, 995-1008.

4.     Bailong Zhang, Chenjiang You, Yong Zhang, Liping Zeng, Jun Hu, Minglei Zhao and Xuemei Chen*. (2020). Linking key steps of microRNA biogenesis by TREX-2 and the nuclear pore complex in Arabidopsis. Nature Plants 6, 957–969.

5.     Jianbo Song, Xiaoyan Wang, Bo Song, Lei Gao, Xiaowei Mo, Luming Yue, Haiqi Yang, Jiayun Lu, Guodong Ren, Beixin Mo* and Xuemei Chen*. (2019). Prevalent cytidylation and uridylation of precursor miRNAs in Arabidopsis. Nature Plants 5, 1260-1272.

6.     Shengben Li, Brandon Le, Xuan Ma, Shaofang Li, Chenjiang You, Lin Liu, Lei Gao, Ting Shi, Yonghui Zhao, Beixin Mo, Xiaofeng Cao, and Xuemei Chen*. (2016). Biogenesis of phased siRNAs on membrane-bound polysomes in Arabidopsis. eLife 2016;5:e22750. 

7.     Yuanyuan Zhao, Yu Yu, Jixian Zhai, Vanitharani Ramachandran, Thanh Theresa Dinh, Blake C. Meyers, Beixin Mo*, and Xuemei Chen*. (2012). The Arabidopsis nucleotidyl transferase HESO1 uridylates unmethylated small RNAs to trigger their degradation. Current Biology 22, 689-694.

8.     Vanitharani Ramachandran and Xuemei Chen*. (2008). Degradation of microRNAs by a family of exoribonucleases in Arabidopsis. Science 321, 1490-1492.

9.     Junjie Li, Zhiyong Yang, Bin Yu, Jun Liu, and Xuemei Chen*. (2005). Methylation protects miRNAs and siRNAs from a 3’ end uridylation activity in Arabidopsis. Current Biology 15, 1501-1507.

10.   Bin Yu, Zhiyong Yang, Junjie Li, Svetlana Minakhina, Maocheng Yang, Richard W. Padgett, Ruth Steward, and Xuemei Chen*. (2005). Methylation as a crucial step in plant microRNA biogenesis. Science 307, 932-935. 

11.    Xuemei Chen*. (2004). A microRNA as a translational repressor of APETALA2 in Arabidopsis flower development. Science 303, 2022-2025. 

12.   Wonkeun Park, Junjie Li, Rentao Song, Joachim Messing, and Xuemei Chen*. (2002). CARPEL FACTORY, the Dicer homologue, and HEN1, a novel protein, act in microRNA metabolism in Arabidopsis thaliana. Current Biology 12, 1484-1495. 

Selected papers on noncanonical RNA capping 

13.   Hao Hu, Nora Flynn, Hailei Zhang, Chenjiang You, Runlai Hang, Xufeng Wang, Huan Zhong, Zhulong Chan, Yiji Xia*, and Xuemei Chen*. (2021). SPAAC-NAD-seq, a sensitive and accurate method to profile NAD+-capped transcripts. PNAS 118 (13) e2025595118. 

14.   Hailei Zhang, Huan Zhong, Xufeng Wang, Shoudong Zhang, Xiaojian Shao, Hao Hu, Zhiling Yu, Zongwei Cai*, Xuemei Chen* and Yiji Xia*. (2021). Use of NAD tagSeq II to identify growth-phase dependent alterations in E. coli RNA NAD+-capping. PNAS 118 (14) e2026183118.

15.   Hailei Zhang, Huan Zhong, Shoudong Zhang, Xiaojian Shao, Min Ni, Zongwei Cai, Xuemei Chen*, and Yiji Xia*. (2019). NAD tagSeq revealed that NAD⁺-capped RNAs are produced from a large number of protein-coding genes in Arabidopsis. PNAS 116, 12072-12077. 

16.  Yuan Wang, Shaofang Li, Yonghui Zhao, Chenjiang You, Brandon Le, Zhizhong Gong, Beixin Mo, Yiji Xia and Xuemei Chen*. (2019). NAD⁺-capped RNAs are widespread in the Arabidopsis transcriptome and can probably be translated. PNAS 116, 12094-12102.