秦为
邮箱: weiqin@mail.tsinghua.edu.cn
课题组主页thu-qin-lab.org
秦为课题组将围绕蛋白质动态调控，翻译后修饰和生物大分子相互作用等重要生物学问题，进一步发展功能驱动的，具有时空动态分辨率的的新型化学工具和化学蛋白质组学技术。具体研究大方向如下：
1. 发展新型化学蛋白质组学策略研究蛋白质的时空调控机制，包括蛋白质空间转运，瞬时蛋白构型变化，蛋白合成与降解等。
2. 发展新型化学蛋白质组学策略研究蛋白-RNA相互作用，蛋白-蛋白相互作用，蛋白-代谢物相互作用等等。并将其应用于解析宿主病原体相互界面中的潜在调控机制。
3. 大规模分析蛋白翻译后修饰的动态调控，包括糖基化, 衣康酸修饰等, 并进一步挖掘宿主病原体相互界面中的新型翻译后修饰。
代表性论文：
(1) Wei Qin#; Joleen S. Cheah#; Charles Xu; James Messing; Brian D. Freibaum; Steven
Boeynaems; J. Paul Taylor; Namrata D. Udeshi; Steven A. Carr; Alice Y. Ting ; Dynamic mapping of proteome trafficking within and between living cells by TransitID, BioRxiv. 2023.
(2) Wei Qin; Samuel A. Myers; Dominique K. Carey; Steven A. Carr; Alice Y. Ting ;
Spatiotemporally-resolved mapping of RNA binding proteins via functional proximity labeling
reveals a mitochondrial mRNA anchor promoting stress recovery, Nature Communications, 2021,12(1).
(3) Wei Qin#; Yanling Zhang#; Huan Tang; Dongyang Liu; Ying Chen; Yuan Liu; Chu Wang ;
Chemoproteomic Profiling of Itaconylation by Bioorthogonal Probes in Inflammatory Macrophages, Journal of the American Chemical Society, 2020, 142(25): 10894-10898.
(4) Wei Qin; Ke Qin; Yanling Zhang; Wentong Jia; Ying Chen; Bo Cheng; Linghang Peng; Nan
Chen; Yuan Liu; Wen Zhou; Yan-Ling Wang; Xing Chen; Chu Wang ; S-glycosylation-based cysteine profiling reveals regulation of glycolysis by itaconate, Nature Chemical Biology, 2019, 15(10): 983-991.
(5) Wei Qin#; Ke Qin#; Xinqi Fan; Linghang Peng; Weiyao Hong; Yuntao Zhu; Pinou Lv; Yifei Du; Rongbing Huang; Mengting Han; Bo Cheng; Yuan Liu; Wen Zhou; Chu Wang; Xing Chen ; Artificial Cysteine S-Glycosylation Induced by Per-O-Acetylated Unnatural Monosaccharides during Metabolic Glycan Labeling, Angewandte Chemie International Edition, 2018, 57(7): 1817-1820.
(6) Wei Qin; Pinou Lv; Xinqi Fan; Baiyi Quan; Yuntao Zhu; Ke Qin; Ying Chen; Chu Wang; Xing
Chen ; Quantitative Time-resolved Chemoproteomics Reveals that Stable O-GlcNAc Regulates Box C/D snoRNP Biogenesis, Proceedings of the National Academy of Sciences of the United States of America, 2017, 114(33): 6749-6758.