学术论文
Li ZJ, Shi ZY, Jian J, Guo YY, Wu Q and Chen GQ. Production of Poly(3-hydroxybutyrate-co-4-hydroxybutyrate) from Unrelated Carbon Sources by Metabolically Engineered Escherichia coli. METABOLIC ENGINEERING 12(4): 352-359, 2010
发布时间：2011-11-16作者：陈国强关键字：
| Abstract A metabolically engineered Escherichia coli has been constructed for the production of poly(3-hydroxybutyrate-co-4-hydroxybutyrate) [P(3HB-co-4HB)] from unrelated carbon sources. Genes involved in succinate degradation in Clostridium kluyveri and P(3HB) accumulation pathway of Ralstonia eutropha were co-expressed for the synthesis of the above copolyester. E. coli native succinate semialdehyde dehydrogenase genes sad and gabD were both deleted for eliminating succinate formation from succinate semialdehyde, which functioned to enhance the carbon flux to 4HB biosynthesis. The metabolically engineered E. coli produced 9.4 gl(-1) cell dry weight containing 65.5% P(3HB-co-11.1 mol% 4HB) using glucose as carbon source in a 48 h shake flask growth. The presence of 1.5-2 gl(-1) alpha-ketoglutarate or 1.0 gl(-1) citrate enhanced the 4HB monomer content from 11.1% to more than 20%. In a 6l fermentor study, a 23.5 gl(-1) cell dry weight containing 62.7% P(3HB-co-12.5 mol% 4HB) was obtained after 29 h of cultivation. To the best of our knowledge, this study reports the highest 4HB monomer content in P(3HB-co-4HB) produced from unrelated carbon sources. |
上一篇：Han ZF, Niu TH, Chang JB, Lei XG, Zhao MY, Wang Q, Cheng W, Wang JJ, Feng Y, Chai JJ. Crystal st...
下一篇：Peng SW, Guo XY, Shang GG, Li J, Xu XY, You ML, Li P, Chen GQ. An Assessment of the Risks of Carc...