Wei Qin, PhD

Email: weiqin@mail.tsinghua.edu.cn

Group website: thu-qin-lab.org

The Qin Lab is long dedicated to developing function-driven chemical biology tools and proteomics technologies with spatiotemporal dynamic resolution, to explore critical biological questions such as intercellular communication, organelle and biomolecular interactions, and post-translational modifications. Specific research directions include:

1.Developing novel in vivo-compatible proximity labeling technologies to achieve tissue- and cell-specific spatiotemporal proteomics in vivo, and to explore spatiotemporal regulatory mechanisms such as intercellular protein communication, transient protein conformational changes, and protein synthesis and degradation.

2.Developing novel chemical proteomics strategies to study organelle interactions and protein interactions with other biomolecules, and applying these strategies to elucidate potential regulatory mechanisms in neurodegenerative diseases.

3.Conducting large-scale analysis of the dynamic regulation of post-translational modifications, and further mining novel post-translational modifications and their regulatory mechanisms.

Selected Publications:

1.Lu, W.#; Zhang, Y.#; Wang, P.; Ni, X.; Zhuang, S.*; Qin, W.*. Spatiotemporal profiling of modification-specific proteome secretion uncovers an itaconation-activated tyrosine kinase. Nat Commun. Revised. ( # co-first authors; * co-corresponding authors)

2.Huang, M.#; Fu, J.#; Wang, P.#; Chen, B.#; Yuan, Q.#; Yu, J.; Wang, H.; Liu, Y.; Li, Z.; Wu, Y.; Ying, T.; Wu, Q.; Zhu, M.*; Qin, W.*; Li, Y.*. Nearly complete conversion of insertion-type indel enhances knock-in and facilitates endogenous biomolecular condensate analysis. Submitted. Cell Research. In press. (# co-first authors; * co-corresponding authors)

3.Wang, W.#; Guo, H.#; Yan, X.#; Pan, X.; Wang, X.; Rong, Y.; Bai, Z.; Zhang, L.; Wu, Z.; Zhao, X.; Huang, W.; Qin, W.*; Chu, L.*. Silicon-rhodamine-enabled Identification (SeeID) for near-infrared light-controlled proximity labeling in vitro and in vivo. Nat Commun. 2025. (# co-first authors; * co-corresponding authors)

4.Sun, X.#; Zhang, Y.#; Lu, W.#; Guo, H.#; He, G.; Luo, S.; Guo, H.; Zhang, Z.; Wang, W.; Chu, L.; Liu, X.; Qin, W.*, Precise and in vivo-compatible spatial proteomics via bioluminescence triggered photocatalytic proximity labeling. ACS Cent. Sci. 2025. (# Equal Contribution)

5.Zhang, ZJ.#; Wang, YK.#; Lu, WJ.; Wang, XF.; Guo, HY.; Pan, XZ.; Liu, ZY.; Wu, ZF.; Qin, W.*, Spatiotemporally-resolved mapping of extracellular proteomes via in vivo-compatible TyroID. Nat. Commun. 2025. (# Equal Contribution)

6.Sun, XG.#; Chen, Y.#; Yang, C.; Yang, S.; Lin, W.; Quan, BY.; Ding, Q.; Chen, X.*; Wang, C.*; Qin, W.*, Chemical recording of pump-specific drug efflux in living cells. Angew. Chem. Int. Ed. 2024. Sep 26:e202409282. (# Equal Contribution)

7.Qin, W.#; Cheah, J.S.#; Xu, C.; Messing, J., Freibaum, B.D., Boeynaems, S., Taylor, J.P., Udeshi, N.D., Carr, S.A., Ting, A.Y. Dynamic mapping of proteome trafficking within and between living cells by TransitID. Cell. 2023, 186 (15), 3307-3324. (# Equal Contribution)

8.Qin, W.; Samuel, M.A.; Carey, C.K.; Carr, S.A.; Ting, A.Y. Spatiotemporally-resolved mapping of RNA binding proteins via functional proximity labeling reveals a mitochondrial mRNA anchor promoting stress recovery. Nat. Commun. 2021, 12 (1), 4980.

9.Qin, W. #; Cho, F.K. #; Cavanagh, P.E. #; Ting, A.Y. Deciphering Molecular Interactions by Proximity Labeling. Nat. Methods. 2021, 18 (2), 133-143. (# Equal Contribution)

10.Qin, W.#; Zhang, Y.#; Tang, H.; Liu D.; Chen, Y.; Liu, Y.; Wang, C. Chemoproteomic Profiling of Itaconylation by Bioorthogonal Probes in Inflammatory Macrophages. J. Am. Chem. Soc. 2020, 142 (25), 10894-10898. (# co-first authors)

11.Qin, W.; Qin, K.; Zhang, Y.; Jia, W.; Chen, Y.; Cheng, B.; Peng, L.; Chen, N.; Liu, Y.; Zhou, W.; Wang, YL.; Chen, X.*; Wang, C.*. S-glycosylation-based cysteine profiling reveals regulation of glycolysis by itaconate. Nat. Chem. Biol. 2019, 15 (10), 983-991. (* co-corresponding authors)

12.Qin, W.#; Qin, K.#; Fan, X.; Peng, L.; Hong, W.; Zhu, Y.; Lv, P.; Du, Y.; Huang, R.; Han, M.; Cheng, B.; Liu, Y.; Zhou, W.; Wang, C.*; Chen, X.*. Artificial Cysteine S-Glycosylation Induced by Per-O-Acetylated Unnatural Monosaccharides during Metabolic Glycan Labeling. Angew. Chem. Int. Ed. 2018, 57 (7), 1817-1820. ( # co-first authors; * co-corresponding authors) (Selected as back cover and very important paper)

13.Qin, W.; Lv, P.; Fan, X.; Quan, B.; Zhu, Y.; Qin, K.; Chen, Y.; Wang, C.*; Chen, X.*. Quantitative Time-resolved Chemoproteomics Reveals that Stable O-GlcNAc Regulates Box C/D snoRNP Biogenesis. Proc. Natl. Acad. Sci. USA 2017, 114 (33), E6749-e6758. (* co-corresponding authors)