Xueming Li, Ph.D,

Tenure-track assistant professor

1998-2002      B.S. University of Science and Technology Beijing

2002-2005      M.S. University of Science and Technology Beijing

2005-2009      Ph.D. Institute of Physics, CAS

2009-2013      Postdoc University of California, San Francisco

2014-2017       Assistant professor (Tenure-track), Tsinghua University

2017-2019       Associate professor(Tenure-track), Tsinghua University

2019-present   Associate professor(Tenured), Tsinghua University

Main Research Fields:

Xueming Li engaged in theoretical and methodological research on cryo-electron microscopy (CryoEM) and its applications in structural biology to investigate fundamental biological questions. Our work focuses on developing and implementing novel image processing techniques and experimental approaches to enhance CryoEM's capability for routine atomic-resolution structural analysis of biological specimens. Looking ahead, Xueming Li aim to develop next-generation cryo-electron microscopy technologies to overcome current technical limitations, enabling the visualization of nanometer-scale and ultimately atomic-resolution structures within tissues and cells. These advancements will facilitate in situ understanding of fundamental life processes and expand the application of cryo-electron microscopy technology to broader fields, including pharmaceutical design and medical research.

Selected Publications:

1.Ranhao Zhang#, Yuan Shen*, Xueming Li*. Fourier-based multiple-slice reconstruction in cryo-electron tomography. Ultramicroscopy, 2025, 277, 114223.

2.Mingtao Huang#, Jinying Ma, Xiaoyu Fu, Hongwei Wang, Yuan Shen*, Xueming Li*. Accurate helical parameter estimation based on cylindrical unrolling. Structure, 2025, 33, 1603-13.

3.Xiaofeng Yan#, Shudong Li, Weilin Huang, Hao Wang, Tianfang Zhao, Mingtao Huang, Niyun Zhou, Yuan Shen, Xueming Li*. MPicker: Visualizing and Picking Membrane Proteins for Cryo-Electron Tomography. Nature Communications, 2025, 16, 472.

4.Ranhao Zhang#, Yuan Shen*, Xueming Li*. Tilt-series-based joint CTF estimation for cryo-electron tomography. Structure, 2024, 32, 1239-47.

5.Chao Lin#, Li Zhang#, Ziying Zhang#, Yifeng Jiang#, Xueming Li*. Locating cellular contents during cryoFIB milling using cellular secondary-electron imaging. Journal of Structural Biology, 2023, 215, 108005.

6.Sihan Wang#, Heng Zhou#, Wei Chen#, Yifeng Jiang, Xuzhen Yan, Hong You*, Xueming Li*. CryoFIB milling large tissue samples for cryo-electron tomography. Scientific Reports, 2023,13, 5879.

7.Xinyu Zhang#, Tianfang Zhao#, Jiansheng Chen*, Yuan Shen*, Xueming Li*, EPicker is an exemplar-based continual learning approach for knowledge accumulation in cryoEM particle picking, Nature Communications, 2022, 13, 2468

8.Xuzhong Yang#, Chao Lin#, Xudong Chen#, Shouqin Li#, Xueming Li*, Bailong Xiao*, Structure deformation and curvature sensing of PIEZO1 in lipid membranes, Nature, 2022, 604, 377-383

9.Mingxu Hu#, Qi Zhang#, Jing Yang*, Xueming Li*，Unit quaternion description of spatial rotations in 3D electron cryo-microscopy, Journal of Structural Biology, 2020, 212, 107601

10.Heng zhou#, Feng Luo#, Zhipu Luo#, Dan Li, Cong Liu*, Xueming Li*, Programming conventional electron microscopes for solving ultrahigh-resolution structures of small and macro-molecules, Analytical Chemistry, 2019, 91, 10996-11003

11.Meijing Li#, Xian Xia#, Yuanyuan Tian#, Qi Jia#, Xiaoyu Liu#, Ying Lu, Ming Li*, Xueming Li*, and Zhucheng Chen*, Mechanism of DNA translocation underlying chromatin remodeling by Snf2, Nature, 2019, 567, 409-413

12.Heng Zhou#, Zhipu Luo#, Xueming Li*, Using Focus Ion Beam to Prepare Crystal Lamella for Electron Diffraction, Journal of Structural Biology, 2019, 205, 59-64

13.Mingxu Hu#, Hongkun Yu#, Kai Gu#, Zhao Wang, Huabin Ruan, Kunpeng Wang, Siyuan Ren, Bing Li, Lin Gan, Shizhen Xu, Guangwen Yang*, Yuan Shen*, Xueming Li*, A particle-filter framework for robust cryoEM 3D reconstruction, Nature Methods, 2018, 15, 1083-1089

Contact：

Homepage: http://thuem.net