Ye Xiang

Email：yxiang@.tsinghua.edu.cn

TEL：＋86-10-62772587（Lab）

Research Area

We are interested in the molecular mechanisms involved in virus entry and assembly. In the recent years, we studied the glycoprotein mediated entry of enveloped viruses (PNAS, 2025; Cell Reports, 2025; Nature Commun., 2024; Cell，2023； mBio，2022；Commun. Biology, 2022；Nature，2021；PLoS Path.，2021；Cell Reports，2019；PLoS Path.，2018；Cell Research，2017; Science, 2016); We also determined the structures of different complex viruses (Nature Commun.，2023；JVI，2023；Cell Research，2019；Nature Commun.，2019；PNAS，2019； Nature，2016). These studies could guide the developments of vaccine and anti-viral drugs. Now the research in my lab is focused on understanding the interactions between virus and receptor, developing vaccine antigens and understanding the mechanisms that govern the assembly of complex viruses.

Selected Publications

(1) Zhang, Linjing; Meng, Kaiwen; Xiang, Ye*. Cryo-EM structure of the Rift Valley fever virus envelope protein in complex with a potent neutralization antibody. Proc Natl Acad Sci USA, 2025, 122(51):e2514862122.

(2) Liu, Zhe; Yang, Yaoyu; Mao, Sihong; Wang, Zhaoqi; Zhu, Qiheng; Yuan, Yuyu*; Xiang, Ye*. Extensive cultivation of human gut phages revealing undescribed Bacteroidaceae phages. Gut Microbes, 2025, 17(1):2597614.

(3) Chen, Danyu; Hu, Jiao; Zhu, Mengran; Xie, Yufeng; Yao, Hantian; An, Haoran; Meng, Yumin; Wang, Juanjuan; Huang, Xueting; Liu, Yanni; Shao, Zhujun; Xiang, Ye; Qi, Jianxun*; Gao, Fu George*; Zhang Jingren*. C-reactive protein is a broad-spectrum capsule-binding receptor for hepatic capture of blood-borne bacteria. EMBO J., 2025, 44(24):7364-7394.

(4) Wang, Deiping; Zhao, Rong; Hu, Wenshu; Li, Haining; Cao, Jimin*; Zhou, Xin*; Xiang, Ye*. Structural insights into polymerase-catalyzed FAD capping of hepatitis C virus RNA. Nature Communications, 2025, 16(1):7298.

(5) Ma, Bingting; Cao, Ziyi; Ding, Weijia; Zhang, Xinzheng *; Xiang, Ye*; Cao, Duanfang*. Structural basis for the recognition of two different types of receptors by Western equine encephalitis virus. Cell Reports, 2025, 44(6):115724.

(6) Li, Haining; Shao, Luyuan; Liu, Zhe; Liu, Qi*; Xiang, Ye*. Molecular mechanisms of the viral encoded chaperone 100K in capsid folding and assembly of adenovirus. Nature Communications, 2025, 16(1):4013.

(7) Wang, Wen; Mohammed, Abdulmelik; Chen, Rong; Elonen, Antti; Chen, Silian; Tian, Mengfan; Yang, Junhao; Xiang, Ye*; Orponen, Pekka*; Wei, Bryan*. Automated design of scaffold-free DNA wireframe nanostructures. Nature Communications, 2025, 16(1):4666.

(8) Zhang, Fan; Chen, Haoze; Zheng, Bo; Huang, Liang; Xiang, Ye; Zhang, Jingren; Liu, Jiafeng*. Heat stress induces phage tolerance in Enterobacteriaceae. Elife, 2025, 14:RP105703.

(9) Li, Ruofan; Gao, Jingjing; Wang, Lin; Gui, Miao*; Xiang, Ye*. Multivalent interactions between fully glycosylated influenza virus hemagglutinins mediated by glycans at distinct N-glycosylation sites. npj Viruses, 2024, 2:48.

(10) Cao, Duanfang; Ma, Bingting; Cao, Ziyi; Xu, Xiaoyu; Zhang, Xinzheng*; Xiang, Ye*. The receptor VLDLR binds Eastern Equine Encephalitis virus through multiple distinct modes. Nature Communications. 2024, 15:6866.

(11) Cao, Duanfang; Ma, Bingting; Cao, Ziyi; Zhang, Xinzheng*; Xiang, Ye*; Structure of Semliki Forest virus in complex with its receptor VLDLR. Cell. 2023, 186(10): 2208-2218.

(12) Jia, Qi; Xiang, Ye*; Cryo-EM structure of a bacteriophage M13 mini variant. Nature Communications, 2023, 14(1): 5421.

(13) Chen, Silian; Rong, Miao; Lv, Yun; Zhu, Deyu*; Xiang, Ye*; Regulation of cGAS activity by RNA‐modulated phase separation. EMBO reports, 2023, 24(2): e51800.

(14) Liu, Zhe; Xiang, Ye*; Structural studies of the nucleus-like assembly of jumbo bacteriophage 201φ2-1. Frontiers in Microbiology, 2023, 14: 1170112.

(15) Li, Haining; Liu, Qi; Shao, Luyuan; Xiang, Ye*; Structural insights into the assembly of the African Swine Fever Virus inner capsid. Journal of Virology, 2023, 97(6): e0026823.

(16) Tong, Liangqin; Wang, Lin; Liao, Shumin; Xiao, Xiaoping; Qu, Jing; Wu, Chunli; Zhu, Yibin; Tai, Wanbo; Huang, Yanhong; Wang, Penghua; Li, Liang; Zhang, Renli; Xiang, Ye*; Cheng, Gong*; A retinol derivative inhibits SARS-CoV-2 infection by interrupting spike-mediated cellular entry. MBio, 2022, 13(4): e0148522.

(17) Ma, Bingting; Huang, Cuiqing; Ma, Jun; Xiang, Ye*; Zhang, Xinzheng*; Structure of Venezuelan equine encephalitis virus with its receptor LDLRAD3. Nature, 2021, 598(7882): 677- 681.

(18) Watson, Avia; Li, Hao; Ma, Bingting; Weiss, Ronen; Bendayan, Daniele; Abramovitz, Lilach; Ben-Shalom, Noam; Mor, Michael; Pinko, Erica; Oz, Michal Bar; Wang, Zhenqi; Du, Fengjiao; Lu, Yu; Rybniker, Jan; Dahan, Rony; Huang, Hairong; Barkan, Daniel; Xiang, Ye*; Javid, Babak*; Freund, Natalia T.*; Human antibodies targeting a Mycobacterium transporter protein mediate protection against tuberculosis. Nature Communications, 2021, 12(1): 602.

(19) Huang, Liang; Xiang, Ye*; Structures of the tailed bacteriophages that infect Gram-positive bacteria. Current Opinion in Virology, 2020, 45: 65-74.

(20) Gao, Jingjing; Gui, Miao*; Xiang, Ye*; Structural intermediates in the low pH-induced transition of influenza hemagglutinin. PLoS Pathogens, 2020, 16(11): e1009062.

(21) Wang, Lin; Wang, Ruoke; Wang, Lei; Ben, Haijing; Yu, Lei; Gao, Fei; Shi, Xuanling; Yin, Chibiao; Zhang, Fuchun; Xiang, Ye*; Zhang, Linqi*; Structural basis for neutralization and protection by a Zika virus-specific human antibody. Cell Reports, 2019, 26(12): 3360-3368.

(22) Wang, Wen; Chen, Silian; An, Byoungkwon; Huang, Kai; Bai, Tanxi; Xu, Mengyuan; Bellot, Gaetan; Ke, Yonggang; Xiang, Ye*; Wei, Bryan*; Complex wireframe DNA nanostructures from simple building blocks. Nature Communications, 2019, 10(1): 1067.

(23) Xu, Jingwei; Dayan, Nir; Goldbourt, Amir*; Xiang, Ye*; Cryo-electron microscopy structure of the filamentous bacteriophage IKe. Proc Natl Acad Sci USA, 2019, 116(12): 5493-5498.

(24) Liu, Qi; Ma, Bingting; Qian, Nianchao; Zhang, Fan; Tan, Xu; Lei, Jianlin; Xiang, Ye*; Structure of the African swine fever virus major capsid protein p72. Cell Research, 2019, 29(11): 953-955.

(25) Xu, Jingwei; Wang, Dianhong; Gui, Miao; Xiang, Ye*. Structural assembly of the tailed bacteriophage phi29. Nature Communications, 2019, 10(1):2366.

(26) Song, Wenfei; Gui, Miao; Wang, Xinquan*; Xiang, Ye*; Cryo-EM structure of the SARS coronavirus spike glycoprotein in complex with its host cell receptor ACE2. PLoS Pathogens, 2018, 14(8): e1007236.

(27) Xu, Jingwei; Xiang, Ye*; Membrane Penetration by Bacterial Viruses. Journal of Virology, 2017, 91(13): e00162-17.

(28) Gui, Miao; Song, Wenfei; Zhou, Haixia; Xu, Jingwei; Chen, Silian; Xiang, Ye*; Wang, Xinquan*; Cryo-electron microscopy structures of the SARS-CoV spike glycoprotein reveal a prerequisite conformational state for receptor binding. Cell Research, 2017, 27(1): 119-129.

(29) Misasi, J; Gilman, MS; Kanekiyo M; Gui, Miao; Cagigi, A; Mulangu, S; Corti, D; Ledgerwood, JE; Lanzavecchia, A; Cunningham, J; Muyembe-Tamfun, JJ; Baxa, U; Graham, BS; Xiang, Ye*; Sullivan, NJ*; McLellan, JS; Structural and molecular basis for Ebola virus neutralization by protective human antibodies. Science, 2016, 351(6279):1343-6.

(30) Xu, Jingwei; Gui, Miao; Wang, Dianhong; Xiang, Ye; The bacteriophage phi29 tail possesses a pore-forming loop for cell membrane penetration. Nature, 2016, 534(7608): 544-547.

(31) Zhu, Deyu; Wang, Lijun; Shang, Guijun; Liu, Xue, Zhu, Jing; Lu, Defeng; Wang, Lei; Kan, Biao; Zhang, Jingren; Xiang, Ye*; Structural biochemistry of a Vibrio cholerae dinucleotide cyclase reveals cyclase activity regulation by folates. Molecular Cell, 2014, 55(6):931-937.