Daochun Kong
Email: kongdc(at)pku(dot)edu(dot)cn；
Telephone: +86-10-62760866；
Lab Homepage: http://www.bio.pku.edu.cn/index.php?id=128&action=showmember&uid=29
Research Area:
We try to elucidate, at the molecular level, the mechanism how genomic stability is maintained in eukaryotic cells. Our current studies mainly focus on the following projects: 1) how eukaryotic DNA replication is initiated and regulated; 2) how replication forks are stabilized by checkpoint control; 3) how checkpoint regulates DNA metabolism (replication, recombination and repair) and cell growth cycle; 4) What is the chromatin struture for a particular region and how the chromatin structure changes during cell differentiation; 5) how a defective DNA metabolism protein causes genomic instability and cancer.
Selected Publications:
1. Jiazhi Hu, Lei Sun, Fenfen Shen, Yufei Chen, Yu Hua, Yang Liu, Mian Zhang, Yiren Hu, Qingsong Wang, Wei Xu, Fei Sun, Jianguo Ji, Johanne M. Murray, Antony M. Carr, and Daochun Kong. The Intra-S Phase Checkpoint Targets Dna2 to Prevent Stalled Replication Forks from Reversing. Cell 149, 1221–1232, June 8, 2012.
2. Lihong Wu1,*, Fang Yang1,*, Yuan Zhang1*, Ling Guan1*, Yu Hua1, Kai Liu1, Di Yao1, Qiong Ye1, Jiazhi Hu1, Peng He1, Eishi Noguchi2, Han Xu1, Ming Yang1, Tianyuan Li1, Joel A. Huberman3, Daochun Kong1§ Sap1/Girdin is required to recruit Cdc18/Cdc6 to chromatin for initiation of DNA replication in fission yeast and human cells. (submitted).
3. Yuan Zhang, Xiong Mengling and Daochun Kong*. Sap1 and Cdc18 participate in the intra-S phase checkpoint activation in the fission yeast Schizosacchromyces pombe. ( in preparation)
4. Qiong Ye & Daochun Kong (2013) The determination of interacting domains between Sap1 and Cdc18 proteins. (in preparation)
5. Ling Gui & Daochun Kong (2013) The identification of DNA replication origins in the fission yeast S. pombe. (in preparation)
6. Bochao Liu, Jiazhi Hu & Daochun Kong (2013). The in vivo evidence for the role of Dna2 and Fen1 in the maturation of Okazaki fragments. (in preparation)
7. Lihong Wu & Daochun Kong (2013). The large-scale identification of the sites of pre-replication complex in human cells. (in preparation)