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Bailong Xiao

Bailong Xiao

Email: xbailong@biomed.tsinghua.edu.cn


Research Interests:

Ion channels are fundamental membrane proteins that regulate the flow of ions across cell membranes and control cell excitability and function. Inappropriate channel activities caused by either mutations or abnormal physiological regulations underlie the etiology of numerous pathologies (collectively known as channelopathies). Thus, an understanding of their structure and function is likely to lead to the development of new therapeutics. Our laboratory is interested in investigating the structure-function relationship, physiological and pathophysiological roles of the so-called sensory ion channels such as temperature-activated Transient Receptor Potential (TRP) channels (thermoTRPs) and the newly identified mechanically activated Piezo channels. These channels can function as receptors for touch stimuli including temperature, chemicals, and mechanical force, and consequently govern the sense of touch. By strategically focusing on characterizing known sensory channels and identifying novel ones, we aim to better understand the molecular and cellular mechanisms of the sense of touch and pain and other related physiological processes such as inflammatory responses with an ultimate goal of identifying novel therapeutics. Techniques employed in our research include molecular biology, biochemistry, single cell or high-throughput calcium imaging, electrophysiology, siRNA or compound library screening, generation and behavioral analysis of transgenic mice, etc. Positions for postdoctoral fellow, graduate student and technician are available in our laboratory. 

Selected Publications

1.   Bertrand Coste (co-first), Bailong Xiao (co-first), Jose S. Santos, Ruhma Syeda, Jorg Grandl, Kathryn S. Spencer, Sung Eun Kim, Manuela Schmidt, Jayanti Mathur, Adrienne E. Dubin, Mauricio Montal, Ardem Patapoutian (2012). Piezo Proteins Are Pore-forming Subunits of Mechanically Activated Channels. Nature2012 Feb 19;483(7388):176-81 PMID:22343900 (Selected by Faculty of 1000Featured by Nature News & Views; TSRI News & Views; Reported by Science Daily)

2.    Bailong Xiao, Bertrand Coste, Jayanti Mathur, Ardem Patapoutian (2011). Temperature-dependent STIM1 activation induces Ca2+ influx and modulates gene expression.Nat Chem Biol. 2011 Jun;7(6):351-8. PMID: 21499266 (Nominated for 2011 signaling breakthroughs of the year by Science Signaling; Featured by Nat Chem Biol.News & Views; Featured by TSRI News & Views; Reported by Science Daily)

3.    Bailong Xiao, Ardem Patapoutian (2011). Scratching the surface: a role of pain-sensing TRPA1 in itch. Nat Neurosci. 2011 May;14(5):540-2. PMID: 21522144 News & Views

4.    Bailong Xiao, Adrienne E. Dubin, Badry Bursulaya, Veena Viswanath, Timothy J. Jegla, Ardem Patapoutian (2008). Identification of Transmembrane Domain Five as a Critical Molecular Determinant of Menthol Sensitivity in Mammalian TRPA1 Channels. J Neurosci. 2008 Sep 24;28(39):9640-51.

5.    Lindsey J. Macpherson (co-first), Bailong Xiao (co-first), Kelvin Y. Kwan (co-first), Matt J. Petrus, Adrienne E. Dubin, SunWook Hwang, Benjamin Cravatt, David P. Corey, Ardem Patapoutian (2007). An Ion Channel Essential for Sensing Chemical Damage. J Neurosci. 2007 Oct 17;27(42):11412-5.

6.    Bailong Xiao, Xixi Tian, Wenjun Xie, Peter P. Jones, Shitian Cai, Xianhua Wang, Dawei Jiang, Lin Zhang, Keyun Chen, Michael P. Walsh, Heping Cheng, and S. R. Wayne Chen (2007). Functional Consequence of PKA-dependent Phosphorylation of the Cardiac Ryanodine Receptor: Sensitization of Store-overload-induced Ca2+release (SOICR). J Biol Chem. 2007 Aug 10.

7.    Bailong Xiao, Guofeng Zhong, Masakazu Obayashi, Dongmei Yang, Michael P. Walsh, Yakhin Shimoni, Heping Cheng, Henk ter Keurs, and S. R. Wayne Chen (2006). Serine-2030, but not Serine-2808, is the Major RyR2 Phosphorylation Site Responding to PKA Activation upon beta-adrenergic Stimulation in Normal and Failing Hearts.Biochem J. 2006 Feb 17. (Commented by editorial)

8.    Bailong Xiao, Ming Tao Jiang, Mingcai Zhao, Dongmei Yang, Cindy Sutherland, F. Anthony Lai, Michael P. Walsh, Heping Cheng, David C. Warltier, and S. R. Wayne Chen (2005). Characterization of a Novel Protein Kinase A Phosphorylation Site, Serine-2030, Reveals No PKA Hyperphosphorylation of the Cardiac Ryanodine Receptor in Canine Heart Failure. Circ Res. 2005 Apr 29;96(8):847-55.

9.    Bailong Xiao, Cindy Sutherland, Michael P. Walsh, S. R. Wayne Chen (2004). Protein Kinase A Phosphorylation at Serine-2808 of the Cardiac Ca2+-Release Channel (Ryanodine Receptor) Does Not Dissociate 12.6-kDa FK506-Binding Protein (FKBP12.6). Circ Res. 2004 Mar 5;94(4):487-95.

10. Bailong Xiao, Haruko Masumiya, Dawei Jiang, Ruiwu Wang, Yoshitatsu Sei, Lin Zhang, Takashi Murayama, Yasuo Ogawa, F. Anthony Lai, Terence Wagenknecht, and S. R. Wayne Chen (2002). Isoform Dependent Formation of Heteromeric Ca2+ Release Channels (Ryanodine Receptors). J Biol Chem. 2002 Nov 1; 277(44): 41778-85. (Selected by Faculty of 1000)

11. Adrienne E. Dubin, Manuela Schmidt, Jayanti Mathur, Matt J. PetrusBailong Xiao, Bertrand Coste, Ardem Patapoutian. Inflammatory signals enhance piezo2-mediated mechanosensitive currents. Cell Rep. 2012 Sep 27;2(3):511-7.Epub 2012 Aug 23. PMID: 22921401

12. Peter P. Jones, Xing Meng, Bailong Xiao, Shitian Cai, Jeff Bolstad, Terence Wagenknecht, Zheng Liu and S. R. Wayne Chen (2008). Localization of PKA phosphorylation site, Ser2030, in the three-dimensional structure of cardiac ryanodine receptor. Biochem J. 2008 Mar 1;410(2):261-70.

13. Xing Meng, Bailong Xiao, Shitian Cai, Xiaojun Huang, Fei Li, Jeff Bolstad, Ramon Trujillo, Judith Airey, S. R. Wayne Chen, Terence Wagenknecht, and Zheng Liu (2007). Three-Dimensional Localization of Serine 2808, a Phosphorylation Site in Cardiac Ryanodine Receptor. J Biol Chem. 2007 Aug 31;282(35):25929-39.

14. Dongmei Yang, Wei-Zhong Zhu, Bailong Xiao, Didier X. P. Brochet, S. R. Wayne Chen, Edward G. Lakatta, Rui-Ping Xiao and Heping Cheng (2007). Ca2+/calmodulin kinase II-dependent Phosphorylation of Ryanodine Receptors Suppresses Ca2+ Sparks and Ca2+ Waves in Cardiac Myocytes. Circ Res. 2007 Feb 16;100(3):399-407.

15. Masakazu Obayashi, Bailong Xiao, Bruno D. Stuyvers, Allen W. Davidoff, Jie Mei, S.R. Wayne Chen, Henk E.D.J. ter Keurs (2006). Spontaneous diastolic contractions and phosphorylation of the cardiac ryanodine receptor at serine-2808 in congestive heart failure in rat. Cardiovasc Res. 2006 Jan;69(1):140-51.

16. Dawei Jiang, Ruiwu Wang, Bailong Xiao, Huihui Kong, Philip Choi, Lin Zhang, and S. R. Wayne Chen (2005). Enhanced Store Overload Induced Ca2+ Release (SOICR) and Channel Sensitivity to Luminal Ca2+ Activation is a Common Defect of RyR2 Mutations Linked to Ventricular Tachycardia and Sudden Death. Circ Res. 2005 Nov 25;97(11):1173-81. (Commented by editorial)

17. Dawei Jiang, Bailong Xiao, Dongmei Yang, Ruiwu Wang, Philip Choi, Lin Zhang, Heping Cheng, and S. R. Wayne Chen  (2004). Ryanodine Receptor Mutations Associated with Ventricular Tachycardia and Sudden Cardiac Death Reduce the Threshold for Store Overload Induced Ca2+ Release (SOICR). Proc Natl Acad Sci U S A. 2004 Aug 31;101(35):13062-7.

18. Dawei Jiang, Bailong Xiao, Xiaoli Li, and S. R. Wayne Chen (2003). Smooth Muscle Tissues Express a Major Dominant-Negative Splice Variant of the Type 3 Ca2+ Release Channel (Ryanodine Receptor) J Biol Chem. 2003 Feb 14; 278(7): 4763-9. (Selected by Faculty of 1000)

19. Haruko Masumiya, Ruiwu Wang , Jing Zhang, Bailong Xiao, S. R. Wayne Chen (2003) Localization of the 12.6-kDa FK506-binding protein (FKBP12.6) binding site to the NH2-terminal domain of the cardiac Ca2+ release channel (ryanodine receptor). J Biol Chem. 2003 Feb 7; 278(6): 3786-92.

20. Dawei Jiang, Bailong Xiao, Lin Zhang, S. R. Wayne Chen (2002). Enhanced Basal Activity of a Cardiac Ca2+ Release Channel (Ryanodine Receptor) Mutant Associated With Ventricular Tachycardia and Sudden Death. Circ. Res. 9; 91(3): 218-25. (Commented by editorial)



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