Junyu Xiao
Email: junyuxiao(at)pku(dot)edu(dot)cn；
Telephone: +86-10-62757054；
Office：College of Life Sciences, Peking University, 100871
Research Area:
A major focus of our research is on novel kinases that function in the secretory pathway. These include the Fam20 kinases, POMK, and other proteins that are predicted to be kinases but remain poorly characterized. Some of these proteins specifically phosphorylate secreted proteins and glycans, and their mutations are associated with a number of human diseases. We seek to understand the function and regulation mechanism of these proteins using a multidisciplinary approaches including biochemistry and X-ray crystallography. Recently, we have also expanded our research projects and studied the fatty acid oxidation related proteins using the cryo-electron microscopy technology. In collaboration with Dr. Ning Gao’s group, we have determined a 4.2 Å structure of the human mitochondrial trifunctional protein. Our results reveal the basic architecture of this important enzyme complex and its membrane association mechanism. In the future, we would like to further characterize other molecular machineries that are involved in the fatty acid oxidation process.
Selected Publications:
1. Liang, K., Li, N., Wang, X., Dai, J., Liu, P., Wang, C., Chen, X.W., Gao, N., Xiao, J.* (2018). Cryo-EM structure of human mitochondrial trifunctional protein. Proc Natl Acad Sci U S A 115:7039-7044.
2. Zhang, H., Zhu, Q., Cui, J., Wang, Y., Chen, M.J., Guo, X., Tagliabracci, V.S., Dixon, J.E., Xiao, J.* (2018). Structure and evolution of the Fam20 kinases. Nature Communications 9:1218.
3. Liu, Y., Bhowmick, T., Liu, Y., Gao, X., Mertens, H.D.T., Svergun, D.I., Xiao, J.*, Zhang, Y.*, Wang, J.H.*, Meijers, R*. (2018). Structural Basis for Draxin-Modulated Axon Guidance and Fasciculation by Netrin-1 through DCC. Neuron. 97(6):1261-1267.
4. Du, S., Qu, L.J., Xiao, J.* (2018). Crystal structures of the extracellular domains of the CrRLK1L receptor-like kinases ANXUR1 and ANXUR2. Protein Sci. 27:886-892.
5. Sun, X., Li, Y., He, W., Ji, C., Xia, P., Wang, Y., Du, S., Li, H., Raikhel, N., Xiao, J.*, Guo, H.* (2017). Pyrazinamide and derivatives block ethylene biosynthesis by inhibiting ACC oxidase. Nature Communications 8:15758.
6. Cui, J., Zhu, Q., Zhang, H., Cianfrocco, M.A., Leschziner, A.E., Dixon, J.E.*, Xiao, J.* (2017). Structure of Fam20A reveals a pseudokinase featuring a unique disulfide pattern and inverted ATP-binding. eLife 6, e23990.
7. Zhu, Q., Venzke, D., Walimbe, A.S., Anderson, M.E., Fu, Q., Kinch, L.N., Wang, W., Chen, X., Grishin, N.V., Huang, N., Yu, L., Dixon, J.E., Campbell, K.P.*, Xiao, J.* (2016). Structure of protein O-mannose kinase reveals a unique active site architecture. eLife 5, e22238.
8. Yu, K., Yu, Y., Tang, X., Chen, H., Xiao, J.*, Su, XD*. (2016). Transcriptome analyses of insect cells to facilitate baculovirus-insect expression. Protein Cell 7:373-82.