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Junyu Xiao


Junyu Xiao


Email: junyuxiao(at)pku(dot)edu(dot)cn

Telephone:  +86-10-62757054;

Office:College of  Life Sciences, Peking University, 100871


Research Area:

Protein phosphorylation is a fundamental mechanism that regulates many physiological processes. More than 500 kinases reside in the human kinome; however, almost all of them function inside the cells to phosphorylate intracellular proteins. On the other hand, many extracellular proteins and proteoglycans are also phosphorylated. This paradox suggests that there are kinases residing in the secretory pathway. Indeed, the Fam20 kinases are emerging as the enzymes responsible for phosphorylating secreted proteins. Mutations in the Fam20 family members cause disorders of biomineralization in humans, highlighting the physiological significance of secreted protein phosphorylation. We will focus on understanding the functional mechanism of these novel secretory pathway kinases. We will rely on X-ray crystallography as our main tool; at the same time pursuing multi-disciplinary research approaches.


Selected Publications:


1. Tagliabracci VS#, Xiao J#, Dixon JE. Phosphorylation of Substrates Destined for Secretion by the Fam20 Kinases. Biochem Soc Trans. 2013 Aug 1;41(4):1061-5. (# Equal contribution).

2. Xiao J, Tagliabracci VS, Wen J, Kim SA, Dixon JE. Crystal Structure of the Golgi Casein Kinase. Proc Natl Acad Sci U S A. 2013 Jun 25;110(26):10574-9.

3. Tagliabracci VS, Engel JL, Wen J, Wiley SE, Worby CA, Kinch LN, Xiao J, Grishin NV, Dixon JE. Secreted Kinase Phosphorylates Extracellular Proteins that Regulate Biomineralization. Science. 2012 Jun 1;336(6085):1150-3. (Highlighted by Faculty of 1000)

4. Guo X, Engel JL, Xiao J, Tagliabracci VS, Wang X, Huang L, Dixon JE. UBLCP1 is a 26S proteasome phosphatase that regulates nuclear proteasome activity. Proc Natl Acad Sci U S A. 2011 Nov 15;108(46):18649-54.

5. Mattoo S, Durrant E, Chen MJ, Xiao J, Lazar CS, Manning G, Dixon JE, and Worby CA. Comparative Analysis of Histophilus somni Immunoglobulin-binding Protein A (IbpA) with Other Fic Domain-containing Enzymes Reveals Differences in Substrate and Nucleotide Specificities. J Biol Chem. 2011 Sep 16;286(37):32834-42.

6. Xiao J, Engel JL, Zhang J, Chen MJ, Manning G, and Dixon JE. Structural and functional analysis of PTPMT1, a phosphatase required for cardiolipin synthesis. Proc Natl Acad Sci U S A. 2011 Jul 19;108(29):11860-5.

7. Chen XW, Leto D, Xiao J, Goss J, Wang Q, Shavit JA, Xiong T, Yu G, Ginsburg D, Toomre D, Xu Z, and Saltiel AR. Exocyst function regulated by effector phosphorylation. Nature Cell Biology. 2011 May;13(5):580-8. (Highlighted by Faculty of 1000)

8. Xiao J, Worby CA, Mattoo S, Sankaran B, and Dixon JE. Structural basis of Fic mediated adenylylation. Nature Structural and Molecular Biology. 2010 Aug;17(8):1004-10. (Highlighted by Faculty of 1000)

9. Xiao J, Chen XW, Davies BA, Saltiel AR, Katzmann DJ, and Xu Z. Structural basis of Ist1 function and Ist1-Did2 interaction in the MVB pathway and cytokinesis. Molecular Biology of the Cell. 2009 Aug;20(15):3514-24.

10. Xiao J, Xia H, Zhou J, Azmi I, Davies BA, Katzmann DJ, and Xu Z. Structural basis of Vta1 function in the multi-vesicular body sorting pathway. Developmental Cell 2008 Jan;14(1):37-49. (Highlighted by Faculty of 1000)

11. Azmi I, Davies BA, Xiao J, Babst M, Xu Z, and Katzmann DJ. ESCRT-III family members stimulate Vps4 ATPase activity directly or via Vta1. Developmental Cell 2008 Jan;14(1):50-61. (Highlighted by Faculty of 1000)

12. Xiao J, Xia H, Yoshino-Koh K, Zhou J, and Xu Z. Structural Characterization of the ATPase Reaction Cycle of Endosomal AAA-Protein Vps4. J Mol Biol. 2007 Nov 30;374(3):655-70.


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