Xueming Li
Email: lixueming@mail.tsinghua.edu.cn
Homepage: em.life.tsinghua.edu.cn
Main Research Fields:
We focus on the theoretical and methodological development of electron cryo-microscopy(CryoEM) and its application in structural biology to address important biological questions. We are particularly interested in developing and applying novel image processing technology to advance CryoEM for routine atomic-resolution structure analysis of biological macromolecules.
Selected Publications:
● Soyeon Park*, Xueming Li*, Homin Kim*, Chingakham Ranjit Singh, Geng Tian, Martin A. Hoyt, Scott Lovell, Kevin P. Battaile, Michal Zolkiewski, Philip Coffino, Jeroen Roelofs, Yifan Cheng, and Daniel Finley, Reconfiguration of the proteasome during chaperone-mediated assembly, Nature, 497, 512-516, 2013. (*co-first author)
● Xueming Li, Paul Mooney, Shawn Zheng, Chris Booth, Michael B. Braunfeld, Sander Gubbens, David A. Agard and Yifan Cheng, Electron counting and beam-induced motion correction enable near atomic resolution single particle cryoEM, Nature Methods, 10, 584-590, 2013.
¶ Featured in Nature Methods News and Views: Robert M. Glaeser (2013) Stroboscopic imaging of macromolecular complexes. Nature Methods 10, 475-476.
¶ Featured in Nature Methods: Method of the Year 2013.
● Xueming Li, Shawn Zheng, Kiyoshi Egami,David A. Agard, Yifan Cheng, Influence of electron dose rate on electron counting images recorded with the K2 camera, J Struct. Biol., 184, 251-260, 2013.
● Patricia Grob, Derek Bean, Dieter Typke, Xueming Li, Eva Nogales, and Robert M. Glaeser, Ranking TEM cameras by their response to electron shot noise, Ultramicroscopy, 133，1-7， 2013
● Shenping Wu, Agustin Avila-Sakar, JungMin Kim, David S. Booth, Charles H. Greenberg, Andrea Rossi, Maofu Liao, Xueming Li, Akram Alian, Sarah L. Griner, Narinobu Juge, Yadong Yu, Claudia M. Mergel, Javier Chaparro-Riggers, Pavel Strop, Robert Tampé, Robert H. Edwards, Robert M. Stroud, Charles S. Craik, Yifan Cheng, Fabs enable single particle cryoEM studies of small proteins, Structure, 20, 582-592, 2012.
● Agustin Avila-Sakar, Xueming Li, Shawn Q. Zheng, and Yifan. Cheng, Recording high-resolution images of two-dimensional crystals of membrane proteins, Chapter 8, Electron crystallography of soluble and membrane proteins, Methods in Molecular Biology, 955, 129- 152, 2012.
● Xueming Li, Nikolaus Grigorieff, Yifan. Cheng, GPU-enabled FREALIGN: accelerating single particle 3D reconstruction and refinement in Fourier space on graphics processors, J Struct Biol., 172, 407-12, 2010
In Physics and material science
● Xueming Li, Fanghua Li, Huiqian Luo, Lei Fang, Hai-hu Wen, Transmission electron microscopy study of one-dimensional incommensurate structural modulation in superconducting oxides Bi2+xSr2-xCuO6+δ (0.1≤x≤0.4), Superconductor Science and Technology, 22, 065003, 2009
● Xueming Li, Fanghua Li, Haifu Fan, A revised version of the program VEC, Chinese Physics B, 18(6), 2459-2463, 2009
● Binghui Ge, Yumei Wang, Xueming Li, Fanghua Li , H.L. Song, H.J. Zhang, A study of an incommensurately modulated structure in Ca0.28Ba0.72Nb2O6 by electron microscopy, Philosophical Magazine Letters, 88, 213 – 217, 2008
● Binghui Ge, Fanghua Li, Xueming Li, Yumei Wang, Zhenhua Chi, Changqing Jin, Study of superstructure II in multiferroic BiMnO3, Chinese Physics B, 17, 3163-3169, 2008
● Xueming Li, Si-ze Yang, Xingfang Wu, Preparation of Silicon Carbide Nitride Films on Si Substrate by Pulsed High-Energy Density Plasma, Journal of University of Science and Technology Beijing, 13(3), 272-276, 200613(3), 272-276, 2006