Wensheng Wei

Email:   wswei (at)pku(dot)edu(dot)cn；

Office: Room 201,College of Life Sciences, Peking University, 100871

Research Area:

Wei group is interested in studying the molecular mechanisms of human diseases, especially the host response to microbial pathogenicity. The combination of forward and reverse genetic means is employed, often in a high-throughput fashion, for the identification of host genes of interest. Efforts have also been put into the development and application of eukaryotic gene editing techniques, such as TALENs and CRISPR/Cas9 systems.

Selected Publications:

1. He R, Peng J, Yuan P, Xu F, Wei W*. (2015) Divergent roles of BECN1 in LC3 lipidation and autophagosomal function. Autophagy (in press).
2. Peng J, Zhou Y, Zhu S and Wei W*. (2015) High-throughput Screens in Mammalian Cells Using the CRISPR-Cas9 System. FEBS Journal, DOI: 10.1111/febs.13251. 
3. Ren Q, Li C, Yuan P, Cai C, Luo G, Zhang L and Wei W*. (2015) A Dual-Reporter System for Real-Time Monitoring and High-throughput CRISPR/Cas9 Library Screening of the Hepatitis C Virus. Sci. Rep. 5, 8865; DOI:10.1038/srep08865.
4. Shen J, Cai C, Yu Z, Pang Y, Zhou Y, Qian L, Wei W* and Huang Y*. (2015) A microfluidic live cell assay to study anthrax toxin induced cell lethality assisted by conditioned medium. Sci. Rep. 5, 8651; DOI:10.1038/srep08651.
5. Yuan P, Zhang H, Cai C, Zhu S, Zhou Y, Yang X, He R, Li C, Guo S, Li S, Huang T, Feng H and Wei W*. (2015) Chondroitin sulfate proteoglycan 4 functions as the cellular receptor for Clostridium difficile toxin B. Cell Res. 25:157-168; published online 30 December 2014. (Cover story)
6. Zhou Y, Zhu S, Cai C, Yuan P, Li C, Huang Y and Wei W*. (2014) High-throughput Screening of a CRISPR/Cas Library for Functional Genomics in Human Cells. Nature 509(7501): 487-91.
7. Qian LL, Cai CZ, Yuan PF, Jeong SY, Yang XZ, Almeida VD, Ernst J, Costa M, Cohen SN, Wei WS*. Bidirectional effect of Wnt signaling antagonist DKK1 on the modulation of anthrax toxin uptake. Sci China Life Sci, 2014, 57: 469–481. (Cover story)
8. Yang J, Zhang Y, Yuan P, Cai C, Ren Q, Guo S, Zhu C, Qi H and Wei W*. (2014) A Complete Decoding of DNA Recognition by TAL Effector RVDs. Cell Res. 24:628-631.
9. Yang J, Yuan P, Wen D , Sheng Y, Zhu S, Yu Y, Gao X and Wei W*. (2013) ULtiMATE system for rapid assembly of customized TAL effectors. PLoS One 2013; 8:e75649. 
10. Wei W, Lu Q., Chaudry J, Leppla S, Cohen SN*. (2006) The LDL receptor-related protein LRP6 mediates internalization and lethality of anthrax toxin. Cell 124, 1141-1154.
11. Wei W, PlovanichJones A, Deng W, Jin Q, Collmer A, Huang H, and He SY*. (2000) The gene coding for the Hrp pilus structural protein is required for type III secretion of Hrp and Avr proteins in Pseudomonas syringae pv. tomato. Proc. Natl. Acad. Sci. USA 97, 2247-52. 
12. Roine E, Wei W^, Yuan J, Nurmiaho-Lassila EL, Kalkkinen N, Romantschuk M, He SY*. (1997) Hrp pilus: an hrp-dependent bacterial surface appendage produced by Pseudomonas syringae pv. tomato DC3000. Proc. Natl. Acad. Sci. USA 94, 3459-64. (^Co-first author)