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Wulan Deng



Wulan Deng


Email: wdeng(at)pku(dot)edu(dot)cn




Research interest:


Transcription regulation; Single molecule imaging

The gene expression programs establish and maintain specific cell states, thus the working mechanisms of key transcription factors are critical for understanding cell fate transition and disease development. Microscopical investigation of transcription events in recent years has revealed new biology about transcription regulation. To study how transcription factor works and how they work differently, our lab takes advantage of single molecule tracking and live cell microscopy to study the super-resolved spatial distribution and fast temporal dynamics of key transcription factors in living cells. We also develop microscopic methods driven by biological questions. In addition, we are interested in the regulation of 3D chromatin organization; the principle of chromatin spatial organization and the involved nuclear factors and DNA elements are not fully understood. In summary, our lab uses a combination of fluorescent microscopy, (epi-)genomics, biochemistry and genome editing approaches, and aims to achieve integrated understanding of transcription regulation events at the dimensions of DNA sequence, epi-genetics, highly-resolved spatial and temporal dynamics.



Selected Publications:


1. Deng W† (co-corresponding author), Shi X, Tjian R, Lionnet T and Singer RH†. CASFISH: CRISPR/Cas9 Mediated in situ Labeling of Genomic Loci in Fixed Cells. Proceedings of the National Academy of Sciences. 2015 Sep 22;112(38):11870-5.


2. Knight SC, Xie L, Deng W, Guglielmi B, Witkowsky L, Bosanac L, Zhang E, Beheiry M, Masson J, Dahan M, Liu Z, Doudna JA and Tjian R. Dynamics of CRISPR-Cas9 Genome Interrogation in Living Cells. Science, 2015 Nov 13;350(6262):823-6


3. Deng W*, Rupon JW*, Krivega I, Breda L, Motta I, Jahn KS, Reik A, Gregory PD, Rivella S, Dean A, Blobel GA. Reactivation of developmentally silenced globin genes by forced chromatin looping. Cell, 2014 Aug 14;158(4):849-60.  (*co-first author)


4. Deng W, Blobel GA. Manipulating nuclear architecture. Curr Opin Genet Dev. 2014 Apr;25:1-7.


5. Deng W, Lee J, Wang H, Miller J, Reik A, Gregory PD, Dean A and Blobel GA. Controlling long range genomic interactions at a native locus by targeted tethering of a looping factor. Cell, 2012 Jun 8;149(6):1233-44.  (Featured by Cell PaperFlick)


6. Deng W, Blobel GA. Do chromatin loops provide epigenetic gene expression states? Curr Opin Genet Dev. 2010 Oct;20(5):548-54.


7. Tripic T*, Deng W* (*co-first author), Cheng Y, Zhang Y, Vakoc CR, Gregory GD, Hardison RC, Blobel GA. SCL and associated proteins distinguish active from repressive GATA transcription factor complexes. Blood. 2009 Mar 5;113(10):2191-201.




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