Web Map For Collection 中文 English
Home About us Organization Principal Investigators Research Education& Training Academic activities Facilities Careers Downloads Contact us
Location:Home - Principal Investigators
Guanxiang Liang


Guanxiang Liang

清华大学生物技术馆 3-306

Email: guanxiangliang@tsinghua.edu.cn

Phone: +86-10-62783887

Research Topics

Virome; Microbiome; Bioinformatics

Research Area

The healthy human body harbors trillions of microorganisms, including bacteria, archaea, fungi, and viruses. These microbes play important roles starting with the birth of human babies, and on throughout life, affecting the digestive system, immune system, nervous system and others.

Our long-term goal is to investigate the interactions between humans and their microbes, and the effects of these interactions on human health. Our research will involve the development and implementation of various biomedical technologies, multi-omics approaches, bioinformatics methodologies, model animals and clinical samples. The long-term outcomes will deliver basic knowledge in microbiome research and provide potential directions for microbiome-based therapies and interventions.

In the short term, we focus on the virome, the viral component of the gut microbiome, in the health and disease.

1)    We will center on the establishment of human virome in the neonates. My research interests focus on the interactions between humans and their microbes that have the positive effects on human health. The gut of healthy human neonates is usually devoid of viruses at birth, but quickly becomes colonized. Our previous research found that the colonization of the viral community in infant gut is stepwise. The first wave of viruses is mainly comprised of temperate bacteriophages induced from pioneer bacteria, and the second phase involves viruses that replicate in human cells, which can be modulated by breastfeeding.

2)    We are interested in the mechanisms of virome perturbations in diseases. Our previous studies documented the dynamics of virome in different diseases, such as IBD and COVID-19. These studies provided potential directions for microbiome-based diagnosis, therapies and interventions.

3)    We are eager to explore the virome “Dark Matter”. Our previous research identified thousands of viral genomes from the virome data that cannot be annotated.

Selected Publications

1. Liang G#, Cobián-Güemes AC, Albenberg L, Bushman FD# The gut virome in inflammatory bowel diseases. Current Opinion Virology. 2021 Dec;51:190-198

2. Merenstein C§, Liang G§ , Whiteside SA, Cobián-Güemes AG, Merlino MS, Taylor LJ, Glascock A, Bittinger K, Tanes C, Graham-Wooten J, Khatib LA, Fitzgerald AS, Reddy S, Baxter AE, Giles JR, Oldridge DA, Meyer NJ, Wherry EJ, McGinniss JE, Bushman FD, Collman RG. (2021) Signatures of COVID-19 Severity and Immune Response in the Respiratory Tract Microbiome. mBio. e0177721.

3. Bushman FD#, Liang G#. (2021) Assembly of the virome in newborn human infants. Current Opinion Virology. 2021 Jun;48:17-22. doi: 10.1016/j.coviro.2021.03.004.

4. Liang G#, Bushman FD#. (2021) The human virome: assembly, composition and host interactions. Nature Review Microbiology. 2021 Aug;19(8):514-527.

5. Liang G, Zhao C, Zhang H, Mattei L, Kessler L, Sherrill-Mix S, Bittinger K, Wu G, Baldassano R, DeRusso P, Ford E, Elovitz M, Gerber J, Kelly A, Zemel A, Bushman FD. (2020) Step-wise assembly of the neonatal virome modulated by breast feeding. Nature 2020 Apr 15;1–5.Z.

6. Liang G, Conrad M, Kelsen J, Kessler L, Breton J, Albenberg L, Marakos S, Galgano A, Devas N, Erlichman J, Zhang H, Mattei L, Bittinger K, Baldassano R, Bushman FD. (2020) The dynamics of the gut virome in very early onset inflammatory bowel disease. Journal of Crohn’s and Colitis jjaa094, doi.org/10.1093/ecco-jcc/jjaa094

7. Malmuthuge N§, Liang G§, Guan LL. (2019) Regulation of rumen development in neonatal ruminants through microbial metagenomes and host transcriptomes. Genome Biology 23;20(1):172. doi: 10.1186/s13059-019-1786-0.

8. Nandi S§, Liang G§, Sindhava V, Basu A, Zhang Y, Angireddy R, Avadhani N, Atchison ML. (2020) YY1 Control of Mitochondrial-Related Genes does not Account for Regulation of Immunoglobulin Class Switch Recombination. European Journal of Immunology doi.org/10.1002/eji.201948385.

9. Aggarwala V§, Liang G§, Bushman FD. (2017) Viral communities of the human gut: metagenomic analysis of composition and dynamics. Mobile DNA 2017 Oct 3;8:12

10. Liang G, Malmuthuge N, Bao H, Griebel PJ, Stothard P, Guan LL. (2016) Transcriptome analysis reveals regional and temporal differences in mucosal immune system development in the small intestine of neonatal calves. BMC Genomics doi: 10.1186/s128640162957y.

11. Liang G, Malmuthuge N, Ren Y, Griebel PJ, Guan LL. (2016) Altered microRNA expression and pre-mRNA splicing events reveal new mechanisms associated with early stage Mycobacterium avium subspecies paratuberculosis infection. Scientific Reports 22;6:24964. doi: 10.1038/srep24964.

12. Liang G, Malmuthuge N, McFadden TB, Bao H, Griebel PJ, Stothard P, Guan LL. (2014) Potential Regulatory Role of MicroRNAs in the Development of Bovine Gastrointestinal Tract during Early Life. PLoS ONE 9(3): e92592. doi:10.1371/journal.pone.0092592

13. Liang G, Malmuthuge N, Guan LL, Griebel PJ (2014) Model systems to analyze the role of miRNAs and commensal microflora in bovine mucosal immune system development. Molecular Immunology doi:10.1016/j.molimm.2014.10.014.

14. Wang D§, Liang G§, Wang B, Sun H, Liu J, Guan LL. (2016) Systematic microRNAome profiling reveals the roles of microRNAs in milk protein metabolism and quality: insights on low-quality forage utilization. Scientific Reports 6:21194

15. Yongjuan Guan§, Liang G§, Hawken PAR, Malecki IA, Cozens G, Vercoe PE, Martin GB, Guan LL (2015) Roles of small RNAs in the effects of nutrition on apoptosis and spermatogenesis in the adult testis. Scientific reports 5: 10372. doi: 10.1038/srep10372.





All right reserved Center For Life Sciences