生命中心陈兴获2022 Horace S. Isbell奖 | ACS年会Highlights!

Horace S. Isbell奖是美国化学会糖化学专业委员会（Division of Carbohydrate chemistry）设立的重要奖项，旨在表彰“在糖化学领域做出卓越和持续高质量贡献的45岁以下科学家”，每年全球范围内仅有一位获奖人。

作为奖项的重要组成部分，每位获奖人都享有在ACS年会上做邀请报告的殊荣。生命中心陈兴在刚刚结束的2022年春季年会上做了题为Chemical tools for labeling, imaging and glycoproteomic profiling of brain glycosylation的主旨报告。

【报告摘要】

The human brain accounts for ~2% of the body weight, but consumes as high as 20% of glucose. As a result, the brain possesses the highest level of glycosylation, such as sialylation and O-GlcNAcylation, among all the organs. The glycans in the brain have been implicated in neural connectivity and neurodegenerative diseases. Aiming to elucidate the functional roles of brain glycosylation, we have developed chemical tools for glycan labeling, imaging, and glycoproteomics. To enable in vivo visualization of the sialoglycans in the mouse brain, we used stealth liposomes to shuttle azidosugars into the brain for metabolic labeling of brain sialoglycans, followed by click-labeling with imaging probes. Termed liposome-assisted bioorthogonal reporter (LABOR), this strategy, for the first time, enable sclick-labeling and imaging of brain sialoglycans in living mice. Furthermore, to implement expansion microscopy (ExM) for brain glycan imaging, we developed click-ExM, which integrates click labeling into EXM to enable super-resolution fluorescence imaging of glycans. We demonstrated click-ExM imaging of sialoglycans in cultured neurons and in brain tissues with super-resolution. Finally, we performed glycoproteomic analysis of protein O-GlcNAcylation in primary neurons, which revealed that O-GlcNAc is enriched at the synapses and regulates synapse activation.

【陈兴个人简介】

陈兴教授现任北京大学化学与分子工程学院院长、生命科学联合中心研究员, ACS Central Science编委。他2002年毕业于清华大学化学系获得学士学位，于2007年在加州大学伯克利分校获得博士学位（导师为Carolyn Bertozzi教授和Alex Zettl教授）。之后在哈佛大学Tim Springer教授研究组完成博士后研究工作，于2010年在北京大学开始了自己的独立职业生涯，并于2016年直接晋升为长聘正教授。陈兴教授的研究重点是综合运用化学方法、生物手段和纳米技术，研究糖基化的生物学功能及其在代谢疾病和癌症等中的调控作用。

他的工作获得了多项奖项的认可，包括张树政糖科学杰出成就奖（2021）、科学探索奖（2020）、Okeanos-CAPA资深研究员奖（2019年）、CCS-RSC青年化学家奖（2018年）、ACS David Y.Gin新研究员奖（2016年）、IGO青年糖科学家奖（2015年）和国家杰出青年科学基金（2014年）。

【陈兴近期代表性成果】

Raman Imaging Shines a Light on Neurodegenerative Disorders

De-en Sun and Xing Chen

ACS Cent. Sci. 2020, 6, 4, 459–460

https://doi.org/10.1021/acscentsci.0c00267

Detecting the Sweet Biomarker on Cancer Cells

Xing Chen

ACS Cent. Sci. 2018, 4, 4, 428–430

https://doi.org/10.1021/acscentsci.8b00156

Protein S-Glyco-Modification through an Elimination–Addition Mechanism

Ke Qin, Hao Zhang, Zhenqi Zhao, and Xing Chen*

J. Am. Chem. Soc. 2020, 142, 20, 9382–9388

https://doi.org/10.1021/jacs.0c02110

Multiphase Coacervates Driven by Electrostatic Correlations

Xu Chen, Er-Qiang Chen*, An-Chang Shi*, and Shuang Yang*

ACS Macro Letters 2021, 10, 8, 1041-1047 (Letter)

Publication Date (Web):July 27, 2021DOI: 10.1021/acsmacrolett.1c00282

9-Azido Analogues of Three Sialic Acid Forms for Metabolic Remodeling of Cell-Surface Sialoglycans

Bo Cheng, Lu Dong, Yuntao Zhu, Rongbing Huang, Yuting Sun, Qiancheng You, Qitao Song, James C. Paton, Adrienne W. Paton, and Xing Chen*

ACS Chem. Biol. 2019, 14, 10, 2141–2147

https://doi.org/10.1021/acschembio.9b00556

Chemoproteomic Profiling of O-GlcNAcylation in Caenorhabditis elegans

Wei Qin, Zhongyun Xie, Jingyang Wang, Guangshuo Ou, Chu Wang*, and Xing Chen*

Biochemistry 2020, 59, 34, 3129–3134

https://doi.org/10.1021/acs.biochem.9b00622

Gap-Junction-Dependent Labeling of Nascent Proteins in Multicellular Networks

Yaya Li, Weibing Liu, Qi Tang, Xinqi Fan, Yi Hao, Ling Gao, Zefan Li, Bo Cheng, and Xing Chen*

ACS Chem. Biol. 2019, 14, 2, 182–185

https://doi.org/10.1021/acschembio.8b01065