陈知行
北京大学未来技术学院研究员/长聘副教授
北大-清华生命科学联合中心PI
电子邮件：zhixingchen（AT）pku.edu.cn
实验室主页：http://zhixingchenlab.mysxl.cn/
研究领域：生物成像荧光探针，化学生物学，分子工程与药物研发
研究兴趣：
致力于从分子视角，融汇有机合成、光谱学和化学生物学等领域的先进技术，推动生物成像探针领域的发展，为生物物理、细胞生物学、代谢生理学和神经生物学等领域的研究提供得心应手的新工具。此外也积极探索分子工程与药物研发交叉领域的新方向。
代表性科研论文：
(1) Zhang, J.; Zhang, K.; Wang, K.; Wang, B.; Zhu, S.; Qian, H.; Ma, Y.; Zhang, M.; Liu, T.; Chen, P.; Shen, Y.; Fu, Y.; Fang, S.; Zhang, X.; Zou, P.; Deng, W.; Mu, Y.; Chen Z.* A Palette of Bridged Bicycle-Strengthened Fluorophores. Nat. Methods. 2025, 22, 1276-1287.
(2) Yang, Z.; Zhang, Y.; Fang, Y.; Zhang, Y.; Du, J.; Shen, X.; Zhang, K.; Zou, P.*; Chen, Z.* Spatially barcoding biochemical reactions using DNA nanostructures unveil a major contact mechanism in proximity labeling. Nat. Chem. Biol. 2025 https://doi.org/10.1038/s41589-025-02086-w.
(3) Saimi, D.; Reymond, L.; Aziz, T.; Shen, X.; Luo Z.; Pi, S.; Liu Y.; Fu S.; Ding S.; Meng A. Chen L.; Jiang H.; Chen Z.* Imaging mitochondrial membrane potential via concentration-dependent fluorescence lifetime changes. Nat. Commun. 2025, 16, 11088.
(4) Ling, J.#; Liu, Y.#; Fu, Y.; Liu, S.; Ding, L.; Huang, Lu.; Xi, P.; Chen, Z.* A gentle palette of plasma membrane dyes.PNAS 2025, 122(29), e2504879122.
(5) Ling, J.#; Zhang, Y.#*; Hei, Y.#; Kompa, J.; Yang, C.; Wang, B.; Zhang, J.; Du, J.; Rudi, T.; Zhang, K.; Sun, J.; Wang, W.; Fabritz, S.; Li, Y.; Deng, W.; Zou, P.; Chen, C.*; Chen, Z.* Thioether editing generally increases the photostability of rhodamine dyes on self-labeling tags. PNAS 2025, 122(30), e2426354122.
(6) Chen, J.; Stephan, T.; Gaedke, F.; Liu, T.; Li, Y. Schauss, A.; Chen, P.; Wulff, V.; Jakobs, S.; Jüngst, C.*; Chen, Z.* An aldehyde-crosslinking mitochondrial probe for STED imaging in fixed cells. PNAS 2024, 121, e2317703121.
(7) Liu, T.#; Kompa, J.#; Ling, J.#; Lardon, N.; Zhang, Y.; Chen, J.; Reymond, L.; Chen, P.; Tran, M.; Yang, Z.; Zhang, H.; Liu, Y.; Pitsch, S.; Zou, P.; Wang, L.; Johnsson, K.; Chen, Z.* Gentle rhodamines for live-cell fluorescence microscopy. ACS Cent. Sci. 2024, 10, 1933-44.
(8) Liu, S.#; Ling, J.#; Chen, P.; Cao, C.; Peng, L.; Zhang, Y.; Ji, G.; Guo, Y.; Chen, P. R.; Zou, P.*; Chen, Z.* Orange/far-red hybrid voltage indicators with reduced phototoxicity enable reliable long-term imaging in neurons and cardiomyocytes. PNAS 2023, 120, e2306950120.
(9) Zhang, Y.#; Yang, C.#; Peng, S.; Ling, J.; Chen, P.; Ma, Y.; Wang, W.; Chen, Z.*; Chen, C.* General Strategy To Improve the Photon Budget of Thiol-Conjugated Cyanine Dyes. J. Am. Chem. Soc. 2023, 145, 4187.
(10) Liu, T.#; Stephan, T.#; Chen, P.; Keller-Findeisen, J.; Chen, J.; Riedel, D.; Yang, Z.; Jakobs, S.; Chen Z.* Multi-color live-cell STED nanoscopy of mitochondria with a gentle inner membrane stain (Cover story). PNAS, 2022, 119, e2215799119.
