秦为
邮箱: weiqin@mail.tsinghua.edu.cn
课题组主页：thu-qin-lab.org
科研兴趣：
秦为课题组长期致力于发展功能驱动的，具有时空动态分辨率的化学生物学工具和蛋白质组学技术，探索细胞间通讯，细胞器和生物分子互作，翻译后修饰等重要生物学问题。具体研究大方向如下：
1.发展活体兼容邻近标记新技术，实现组织和细胞特异性的活体时空蛋白质组学，探索细胞间蛋白质通讯、瞬时蛋白构型变化，蛋白合成与降解等时空调控机制。
2.发展新型化学蛋白质组学策略研究细胞器互作、蛋白质与其它生物分子的相互作用，并将其应用于解析神经退行性疾病中的潜在调控机制。
3.大规模分析蛋白翻译后修饰的动态调控，并进一步挖掘新型翻译后修饰及其调控机制。
代表性论文：
1.Lu, W.#; Zhang, Y.#; Wang, P.; Ni, X.; Zhuang, S.*; Qin, W.*. Spatiotemporal profiling of modification-specific proteome secretion uncovers an itaconation-activated tyrosine kinase. Nat Commun. Revised. ( # co-first authors; * co-corresponding authors)
2.Huang, M.#; Fu, J.#; Wang, P.#; Chen, B.#; Yuan, Q.#; Yu, J.; Wang, H.; Liu, Y.; Li, Z.; Wu, Y.; Ying, T.; Wu, Q.; Zhu, M.*; Qin, W.*; Li, Y.*. Nearly complete conversion of insertion-type indel enhances knock-in and facilitates endogenous biomolecular condensate analysis. Submitted. Cell Research. In press. (# co-first authors; * co-corresponding authors)
3.Wang, W.#; Guo, H.#; Yan, X.#; Pan, X.; Wang, X.; Rong, Y.; Bai, Z.; Zhang, L.; Wu, Z.; Zhao, X.; Huang, W.; Qin, W.*; Chu, L.*. Silicon-rhodamine-enabled Identification (SeeID) for near-infrared light-controlled proximity labeling in vitro and in vivo. Nat Commun. 2025. (# co-first authors; * co-corresponding authors)
4.Sun, X.#; Zhang, Y.#; Lu, W.#; Guo, H.#; He, G.; Luo, S.; Guo, H.; Zhang, Z.; Wang, W.; Chu, L.; Liu, X.; Qin, W.*, Precise and in vivo-compatible spatial proteomics via bioluminescence triggered photocatalytic proximity labeling. ACS Cent. Sci. 2025. (# Equal Contribution)
5.Zhang, ZJ.#; Wang, YK.#; Lu, WJ.; Wang, XF.; Guo, HY.; Pan, XZ.; Liu, ZY.; Wu, ZF.; Qin, W.*, Spatiotemporally-resolved mapping of extracellular proteomes via in vivo-compatible TyroID. Nat. Commun. 2025. (# Equal Contribution)
6.Sun, XG.#; Chen, Y.#; Yang, C.; Yang, S.; Lin, W.; Quan, BY.; Ding, Q.; Chen, X.*; Wang, C.*; Qin, W.*, Chemical recording of pump-specific drug efflux in living cells. Angew. Chem. Int. Ed. 2024. Sep 26:e202409282. (# Equal Contribution)
7.Qin, W.#; Cheah, J.S.#; Xu, C.; Messing, J., Freibaum, B.D., Boeynaems, S., Taylor, J.P., Udeshi, N.D., Carr, S.A., Ting, A.Y. Dynamic mapping of proteome trafficking within and between living cells by TransitID. Cell. 2023, 186 (15), 3307-3324. (# Equal Contribution)
8.Qin, W.; Samuel, M.A.; Carey, C.K.; Carr, S.A.; Ting, A.Y. Spatiotemporally-resolved mapping of RNA binding proteins via functional proximity labeling reveals a mitochondrial mRNA anchor promoting stress recovery. Nat. Commun. 2021, 12 (1), 4980.
9.Qin, W. #; Cho, F.K. #; Cavanagh, P.E. #; Ting, A.Y. Deciphering Molecular Interactions by Proximity Labeling. Nat. Methods. 2021, 18 (2), 133-143. (# Equal Contribution)
10.Qin, W.#; Zhang, Y.#; Tang, H.; Liu D.; Chen, Y.; Liu, Y.; Wang, C. Chemoproteomic Profiling of Itaconylation by Bioorthogonal Probes in Inflammatory Macrophages. J. Am. Chem. Soc. 2020, 142 (25), 10894-10898. (# co-first authors)
11.Qin, W.; Qin, K.; Zhang, Y.; Jia, W.; Chen, Y.; Cheng, B.; Peng, L.; Chen, N.; Liu, Y.; Zhou, W.; Wang, YL.; Chen, X.*; Wang, C.*. S-glycosylation-based cysteine profiling reveals regulation of glycolysis by itaconate. Nat. Chem. Biol. 2019, 15 (10), 983-991. (* co-corresponding authors)
12.Qin, W.#; Qin, K.#; Fan, X.; Peng, L.; Hong, W.; Zhu, Y.; Lv, P.; Du, Y.; Huang, R.; Han, M.; Cheng, B.; Liu, Y.; Zhou, W.; Wang, C.*; Chen, X.*. Artificial Cysteine S-Glycosylation Induced by Per-O-Acetylated Unnatural Monosaccharides during Metabolic Glycan Labeling. Angew. Chem. Int. Ed. 2018, 57 (7), 1817-1820. ( # co-first authors; * co-corresponding authors) (Selected as back cover and very important paper)
13.Qin, W.; Lv, P.; Fan, X.; Quan, B.; Zhu, Y.; Qin, K.; Chen, Y.; Wang, C.*; Chen, X.*. Quantitative Time-resolved Chemoproteomics Reveals that Stable O-GlcNAc Regulates Box C/D snoRNP Biogenesis. Proc. Natl. Acad. Sci. USA 2017, 114 (33), E6749-e6758. (* co-corresponding authors)
