李雪明
博士 副教授
1998-2002年 北京科技大学 料物理系 学士
2002-2005年 北京科技大学 材料物理与化学系 硕士
2005-2009年 中科院物理研究所 凝聚态物理 博士
2009-2013年 加州大学旧金山分校 博士后
2014-2017年 清华大学生命科学学院 Tenure-track助理教授
2017-2019年 清华大学生命科学学院 Tenure-track副教授
2019-至今 清华大学生命科学学院 Tenured副教授
主要科研领域与方向:
主要从事冷冻电子显微学理论和方法研究，重点开展最新电子显微学技术的开发与应用，致力于建立具有普适性的亚细胞结构和生物大分子三维重构方法，以提升在原子尺度上观测和研究生命过程的能力。同时，着力推动电子显微学技术在结构生物学领域的应用，解决与生物大分子结构相关的关键科学问题。未来研究将着眼于发展新一代冷冻电镜技术，实现从组织到细胞等多个尺度的纳米级乃至原子级分辨率结构刻画，为在原子或分子水平上阐释生命活动规律提供技术支撑，并促进冷冻电镜技术在药物研发及临床医学等领域的广泛应用。
Main Research Fields:
Xueming Li engaged in theoretical and methodological research on cryo-electron microscopy (CryoEM) and its applications in structural biology to investigate fundamental biological questions. Our work focuses on developing and implementing novel image processing techniques and experimental approaches to enhance CryoEM's capability for routine atomic-resolution structural analysis of biological specimens. Looking ahead, Xueming Li aim to develop next-generation cryo-electron microscopy technologies to overcome current technical limitations, enabling the visualization of nanometer-scale and ultimately atomic-resolution structures within tissues and cells. These advancements will facilitate in situ understanding of fundamental life processes and expand the application of cryo-electron microscopy technology to broader fields, including pharmaceutical design and medical research.
Selected Publications:
1.Ranhao Zhang#, Yuan Shen*, Xueming Li*. Fourier-based multiple-slice reconstruction in cryo-electron tomography. Ultramicroscopy, 2025, 277, 114223.
2.Mingtao Huang#, Jinying Ma, Xiaoyu Fu, Hongwei Wang, Yuan Shen*, Xueming Li*. Accurate helical parameter estimation based on cylindrical unrolling. Structure, 2025, 33, 1603-13.
3.Xiaofeng Yan#, Shudong Li, Weilin Huang, Hao Wang, Tianfang Zhao, Mingtao Huang, Niyun Zhou, Yuan Shen, Xueming Li*. MPicker: Visualizing and Picking Membrane Proteins for Cryo-Electron Tomography. Nature Communications, 2025, 16, 472.
4.Ranhao Zhang#, Yuan Shen*, Xueming Li*. Tilt-series-based joint CTF estimation for cryo-electron tomography. Structure, 2024, 32, 1239-47.
5.Chao Lin#, Li Zhang#, Ziying Zhang#, Yifeng Jiang#, Xueming Li*. Locating cellular contents during cryoFIB milling using cellular secondary-electron imaging. Journal of Structural Biology, 2023, 215, 108005.
6.Sihan Wang#, Heng Zhou#, Wei Chen#, Yifeng Jiang, Xuzhen Yan, Hong You*, Xueming Li*. CryoFIB milling large tissue samples for cryo-electron tomography. Scientific Reports, 2023,13, 5879.
7.Xinyu Zhang#, Tianfang Zhao#, Jiansheng Chen*, Yuan Shen*, Xueming Li*, EPicker is an exemplar-based continual learning approach for knowledge accumulation in cryoEM particle picking, Nature Communications, 2022, 13, 2468
8.Xuzhong Yang#, Chao Lin#, Xudong Chen#, Shouqin Li#, Xueming Li*, Bailong Xiao*, Structure deformation and curvature sensing of PIEZO1 in lipid membranes, Nature, 2022, 604, 377-383
9.Mingxu Hu#, Qi Zhang#, Jing Yang*, Xueming Li*，Unit quaternion description of spatial rotations in 3D electron cryo-microscopy, Journal of Structural Biology, 2020, 212, 107601
10.Heng zhou#, Feng Luo#, Zhipu Luo#, Dan Li, Cong Liu*, Xueming Li*, Programming conventional electron microscopes for solving ultrahigh-resolution structures of small and macro-molecules, Analytical Chemistry, 2019, 91, 10996-11003
11.Meijing Li#, Xian Xia#, Yuanyuan Tian#, Qi Jia#, Xiaoyu Liu#, Ying Lu, Ming Li*, Xueming Li*, and Zhucheng Chen*, Mechanism of DNA translocation underlying chromatin remodeling by Snf2, Nature, 2019, 567, 409-413
12.Heng Zhou#, Zhipu Luo#, Xueming Li*, Using Focus Ion Beam to Prepare Crystal Lamella for Electron Diffraction, Journal of Structural Biology, 2019, 205, 59-64
13.Mingxu Hu#, Hongkun Yu#, Kai Gu#, Zhao Wang, Huabin Ruan, Kunpeng Wang, Siyuan Ren, Bing Li, Lin Gan, Shizhen Xu, Guangwen Yang*, Yuan Shen*, Xueming Li*, A particle-filter framework for robust cryoEM 3D reconstruction, Nature Methods, 2018, 15, 1083-1089
联系方式/Contact：
Homepage: http://thuem.net
