何爱彬
北京大学未来技术学院 教授
生命科学联合中心PI
电子邮件: ahe(at)pku(dot)edu(dot)cn；
实验室主页:http://aibinlab.org
研究领域：
1. 活体胚胎成像与数字谱系构建“数字胚胎”
2. 单细胞与无细胞表观组学新技术开发与细胞命运精准操控研究
3. 新一代表观液体活检技术开发与临床应用
研究兴趣：
生命发育始于一个受精卵，但单个细胞如何通过连续分裂、迁移、相互作用和命运选择，最终形成复杂的组织与器官，仍是发育生物学中的核心问题。何爱彬实验室希望构建哺乳动物发育过程的“数字胚胎”：通过活体长时程成像记录胚胎中每一个细胞的行为轨迹，并结合单细胞表观组学技术解析细胞命运决定背后的染色质调控状态，从而建立细胞行为、谱系关系、表观遗传状态与最终命运之间的定量联系。围绕这一目标，实验室发展了 CoBATCH、CoTECH、uCoTarget、TACIT 等系列单细胞表观组学技术，用于解析细胞命运建立过程中的表观遗传调控逻辑。进一步地，实验室将“细胞起源追踪”的思想拓展至疾病研究，开发基于血浆无细胞核小体的 cf-EpiTracing 技术，探索利用表观遗传信息追踪疾病组织来源、评估疾病状态和治疗反应。总体而言，实验室致力于通过原创技术发展，连接数字胚胎、表观遗传谱系追踪与新一代液体活检，理解生命发育与疾病起源的基本规律，并推动相关技术的临床转化应用。
代表性科研成果(# ,共同通讯作者)：
1. Chen X, Meng X, Zhang W, Zhang X, Zhang Y, Yang P, Liu Y, Bao F, Li S, Wang J, Yan C, Li C, Zhang L, Hao X, Liu J, Sun J, Wang Z, Tian Y, Zhu L, Hou Y, Liu Z, Li W, Mi L, Qi X, Yue Y, Du P, Chen G, Zheng J#, Dou L#, Jing H#, He A#. Cell-free chromatin state tracing reveals disease origin and therapy responses. Nature. 2026;653:599-610. doi: 10.1038/s41586-026-10224-0
2. Liu M, Yue Y, Chen X, Xian K, Dong C, Shi M, Xiong H, Tian K, Li Y, Zhang QC, He A. Genome-coverage single-cell histone modifications for embryo lineage tracing. Nature. 2025;640:828-839. doi: 10.1038/s41586-025-08656-1
3. Dong C, Meng X, Zhang T, Guo Z, Liu Y, Wu P, Chen S, Zhou F, Ma Y, Xiong H, Shu S, He A. Single-cell EpiChem jointly measures drug-chromatin binding and multimodal epigenome. Nat Methods. 2024;21:1624-1633. doi: 10.1038/s41592-024-02360-0
4. Xiong H, Luo Y, Wang Q, Yu X and He A. Single-cell joint detection of chromatin occupancy and transcriptome enables higher-dimensional epigenomic reconstructions. Nat Methods. 2021, 18(6):652-660.
5. Zhang Y, Li X, Gao S, Liao Y, Luo Y, Liu M, Bian Y, Xiong H, Yue Y#., He A#. Genetic reporter for live tracing fluid flow forces during cell fate regulation in mouse blastocyst development. Cell Stem Cell. 2023; 30(8):1110-1123
6. Yue Y, Zong W, Li X, Li J, Zhang Y, Wu R, Liu Y, Cui J, Wang Q, Bian Y, Yu X, Liu Y, Tan G, Zhang Y, Zhao G, Zhou B, Chen L, Xiao W, Cheng H and He A. Long-term, in toto live imaging of cardiomyocyte behaviour during mouse ventricle chamber formation at single-cell resolution. Nat Cell Biol. 2020;22:332-340.
7. Ai S, Xiong H, Li CC, Luo Y, Shi Q, Liu Y, Yu X, Li C and He A. Profiling chromatin states using single-cell itChIP-seq. Nat Cell Biol. 2019;21:1164-1172.
8. Wang Q, Xiong H, Ai S, Yu X, Liu Y, Zhang J and He A. CoBATCH for High-Throughput Single-Cell Epigenomic Profiling. Mol Cell. 2019.
9. Xiong H, Wang Q, Li CC and He A. Single-cell joint profiling of multiple epigenetic proteins and gene transcription. Sci Adv. 2024;10:eadi3664.
10. Li X, Yue Y, Zhang Y, Liao Y, Wang Q, Bian Y, Na J, He A. Continuous live imaging reveals a subtle pathological alteration with cell behaviors in congenital heart malformation. Fundamental Research, 2022, 2(1): 14-22.
