曾虎

北京大学未来技术学院研究员

生命科学联合中心PI

实验室主页：https://zenghupku.github.io/

电子邮件: huzeng（AT）pku.edu.cn；

研究领域：

基因在特定时间和空间的表达决定细胞的功能和命运，我们实验室致力于建立具有空间、时间和单细胞分辨的测序技术，并应用这些测序技术在时空维度探究基因表达调控和疾病致病机理。具体包括：

1. 开发空间多组学技术；

2. 解析基因表达调控网络；

3. 探究神经系统疾病致病机理。

研究兴趣:

实验室聚焦基因在时空维度上的表达调控及其在疾病中的作用。主要方向包括：开发具有空间、时间和单细胞分辨率的多组学测序技术，实现转录组、翻译组等在同一组织样本中的高通量原位联合检测；利用这些时空多组学数据揭示基因表达在不同调控维度的相互作用，解析基因表达调控网络；将方法学与疾病研究结合，重点研究神经退行性疾病与脑肿瘤，解析基因表达时空异常如何驱动病理表型，并寻找潜在治疗靶点。通过技术开发与生物学问题并重，旨在构建从分子机制到临床转化的研究路径，推动时空组学在基础与转化医学中的应用。

代表性科研成果:

1. J Ren^#, H Zeng^#, J Huang^#, J Tian, M Wu, H Shi, X Sui, C K Wang, H Zhou, Z Tang, S Luo, X Wang*. Spatially resolved in situ profiling of mRNA life cycle at transcriptome scale in intact cells and tissues using STARmap PLUS, RIBOmap and TEMPOmap. Nat. Protoc. (2026) 21, 1629–1661.

2. T Hui^#, J Zhou^#, M Yao, Y Xie, H Zeng*. Advances in spatial omics technologies. Small Methods (2025), e2401171. (Review)

3. H Zeng^#, J Huang^#, J Ren^#, C K Wang, Z Tang, H Zhou, Y Zhou, H Shi, A Aditham, X Sui, H Chen, J A Lo, X Wang*. Spatially Resolved single-cell translatomics at molecular resolution. Science (2023) 380, eadd3067. (Highlighted in the Nature Reviews Genetics and Nature Methods).

4. J Ren^#, H Zhou^#, H Zeng^#, C K Wang, J Huang, X Qiu, X Sui, Q Li, X Wu, Z Lin, J A Lo, K Maher, Y He, X Tang, J Lam, H Chen, B Li, D E Fisher, J Liu, X Wang*. Spatiotemporally resolved transcriptomics reveals the subcellular RNA kinetic landscape. Nat. Methods (2023) 20, 695–705.

5. H Zeng^#, J Huang^#, H Zhou^#, W J. Meilandt, B Dejanovic, Y Zhou, C J. Bohlen, S Lee, J Ren, A Liu, Z Tang, H Sheng, J Liu, M Sheng*, X Wang*. Integrative in situ mapping of single-cell transcriptional states and tissue histopathology in a mouse model of Alzheimer's disease. Nat. Neurosci. (2023) 26, 430–446.

6. H Zeng^#, M Mondal^#, R Song^#, J Zhang, B Xia, M Liu, C Zhu, B He, YQ Gao* and C Yi*. Unnatural cytosine bases recognized as thymines by DNA polymerases via the formation of the Watson-Crick geometry. Angew. Chem. Int. Ed. (2019) 58, 130-133.

7. H Zeng^#, B He^#, C Yi*. Compilation of modern technologies to map genome-wide cytosine modifications in DNA. Chembiochem (2019) 20, 1898-1905. (Review)

8. H Zeng^#, B He^#, B Xia, D Bai, X Lu, J Cai, L Chen, A Zhou, C Zhu, H Meng, Y Gao, H Guo, C He*, Q Dai*, and C Yi*. Bisulfite-Free, Nanoscale Analysis of 5-Hydroxymethylcytosine at Single Base Resolution. J. Am. Chem. Soc. (2018) 140, 13190-13194.

9. H Zeng^#, B He^#, C Yi*, and J Peng*. Liquid biopsies: DNA methylation analyses in circulating cell-free DNA. J. Genet. Genomics (2018) 45, 185-192. (Review)