李寅青
邮箱：yinqingl@tsinghua.edu.cn
研究方向：
细胞的“身份”长期被记录在染色质修饰之中，但这些化学标记是可被反复抹除-重写的，那么在不断翻新的标记体系里，稳态如何被维系？又是什么作用让系统跨过阈值、滑向疾病？为回答这一核心问题，实验室同时发展创新实验技术与计算模型，并聚焦两条互补生物体系：（1）神经细胞的转录-表观遗传稳态：神经元如何在数年乃至数十年内保持活动依赖的基因表达程序？稳态的变化如何导致阿尔茨海默病（AD）和成瘾？（2）T 细胞的命运选择：基因调控稳态的变化如何决定增殖、杀伤与耗竭之间的平衡？能否重新设定 “稳态阈值” ，以增强肿瘤免疫疗效或缓解自身免疫？
我们发展基因组分析、编辑与调控技术，多组学、多模态数据的分析框架，力求描绘出稳态-失稳的能量景观，从数据和实验中解析关键因素，寻找可逆干预窗口，为神经退行性疾病与免疫相关疾病提供新的治疗策略。
实验室主页：
https://web.mit.edu/yinqingl/www/
代表性科研论文：
Zhu M#, Yuan J#, Meng Q#, Yu J#, Xu X#, Xu M, Ren X, Hu Y, Wei G, Jia Z, Yuan G, Zang L, Liu S, Yang Y, Zheng Y, Wang J, Cong T, Xie W, Lan X, Cong L, Ma T, Ding S, Guo W, Zhang X*, Li Y*. Minimizing far-extending chromatin perturbation in genome editing preserves stem cell identity. CELL STEM CELL. 2026 Feb 24:S1934-5909(26)00038-X. doi: 10.1016/j.stem.2026.01.015. Epub ahead of print. PMID: 41742419.
Jia Z, Yu J, Li Y. Protocol to perform assay for chromatin-bound condensates by exploratory sequencing in mouse embryonic stem cells. STAR PROTOCOLS. 2025 Sep 19;6(3):104067. doi: 10.1016/j.xpro.2025.104067. Epub 2025 Sep 10. PMID: 40934074; PMCID: PMC12491159.
Huang M#, Fu J#, Wang P#, Chen B#, Yuan Q#, Yu J, Wang H, Liu Y, Li Z, Wu Y, Ying T, Wu Q, Zhu M*, Qin W*, Li Y*. Nearly complete redirection of insertion-type indel into recombination enhances knock-in and facilitates endogenous biomolecular condensate analysis. CELL RESEARCH. 2025 Oct 29. doi: 10.1038/s41422-025-01190-9. Epub ahead of print. PMID: 41152564.
Xu J#, Jia Z#, Zhao X#, Wang L, Jin G, Li Z, Yin N, Li Y*, Peng M*. BCOR and ZC3H12A suppress a core stemness program in exhausted CD8+ T cells. JOURNAL OF EXPERIMENTAL MEDICINE. 2025 Aug 4;222(8):e20241133.
Zhu M#, Xu R#, Yuan J#, Wang J#, Ren X, Cong T, You Y, Ju A, Xu L, Wang H, Zheng P, Tao H, Lin C, Yu H, Du J, Lin X, Xie W, Li Y*, Lan X*. Tracking-seq reveals the heterogeneity of off-target effects in CRISPR-Cas9-mediated genome editing. NATURE BIOTECHNOLOGY. 2025 May;43(5):799-810.
He J#, Huo X#, Pei G#, Jia Z#, Yan Y, Yu J, Qu H, Xie Y, Yuan J, Zheng Y, Hu Y, Shi M, You K, Li T, Ma T, Zhang MQ, Ding S, Li P*, Li Y*. Dual-role transcription factors stabilize intermediate expression levels. CELL. 2024 May 23;187(11):2746-2766.e25.
Li Y#, Lopez-Huerta VG#, Adiconis X#, Levandowski K, Choi S, Simmons SK, Arias-Garcia MA, Guo B, Yao AY, Blosser TR, Wimmer RD, Aida T, Atamian A, Naik T, Sun X, Bi D, Malhotra D, Hession CC, Shema R, Gomes M, Li T, Hwang E, Krol A, Kowalczyk M, Peça J, Pan G, Halassa MM, Levin JZ, Fu Z, Feng G. Distinct subnetworks of the thalamic reticular nucleus. NATURE. 2020, 583(7818):819-824.
Habib N#, Li Y#, Heidenreich M, Swiech L, Avraham-Davidi I, Trombetta JJ, Hession C, Zhang F, Regev A. Div-Seq: Single-nucleus RNA-Seq reveals dynamics of rare adult newborn neurons. SCIENCE. 2016, 353(6302):925-8.
