Dr. Jingyue Ju，Professor，Samuel Ruben-Peter G. Viele Professor of Engineering, Columbia University
“Molecular Engineering Approaches for DNA Sequencing By Synthesis”
Time: 16:00, September 3 (Monday)
Place: Medical Science Building B321
Host: Jingren Zhang
Abstract: DNA sequencing by synthesis (SBS) during polymerase reaction offers a robust platform to decipher DNA sequences. Based on this platform, we are pursuing the research and development of several SBS approaches using molecular engineering. In one method, four nucleotides (A, C, G, T) are modified as nucleotide reversible terminators (NRT) by attaching a cleavable fluorophore to the base and capping the 3’-OH group with a small reversible moiety so that they are still recognized by DNA polymerase as substrates. DNA templates consisting of homopolymer regions were accurately sequenced by using these molecules on a 4-color DNA sequencer. This general strategy to rationally design cleavable fluorescent NRTs for SBS has been widely used in the next generation DNA sequencing systems. We are currently extending the fluorescent NRT based SBS to a single-molecule detection level. To increase the readlength of SBS, we have developed a walking strategy using a mixture of dNTPs/NRT, which can extend the readlength of fluorescent SBS two to three-fold. We have designed and synthesized a novel set of nucleotide analogues that have the potential to enable real time single molecule SBS with electronic detection. Progress from these several areas of research will be presented.
Light Refreshments will be served!!!
Background
The Centre for Infectious Diseases Research is holding a weekly seminar series. The seminars will be a great opportunity to hear speakers from both within and outside China speaking on a variety of subjects of interest to all those engaged in microbiology/immunology and allied disciplines. The coordinator of the seminar series is Babak Javid, and he would be delighted for fellow faculty to get in touch with regards to possible speakers to invite.The seminars will also be of great interest to graduate students in life sciences, so we would appreciate PIs encouraging their students and post-docs to attend. By way of encouragement, we will be providing light refreshments to attendees.If you are interested in meeting with the speaker, please email Babak Javid at bjavid@gmail.com.We hope that this seminar series will prove to be a valuable addition for both training and scientific dialogue at Tsinghua, and look forward to seeing you there.
