时间：2024年11月28日 16:00-17:30

主讲人：杜鹏

主题：miRNA剂量调控的理论和应用

会议地点：清华大学生物新馆143室

邀请人：周帆

个人简介：

杜鹏，北京大学生命科学学院教授，北京大学生命联合中心、北京大学核糖核酸北京研究中心研究员。2012年获北京大学博士学位，博士后工作于美国哈佛大学医学院/波士顿儿童医院, 主要从事干细胞和转化医学中RNA生物学相关研究。最近，实验室利用RNA剪接抑制策略首次实现了小鼠和人类全能卵裂球样细胞的稳定培养；发现了miRNA剂量调控机制，并利用植物免疫蛋白RDR修复了肿瘤miRNA的缺陷，实现了广谱抗肿瘤反应。以通讯作者，分别在Cell, Nature, Molecular Cell和Cell Stem Cell等杂志上发表多篇论文。并获得国家杰出青年科学基金, 基金委原创探索计划, 北京市卓越青年计划等项目支持。现任中国细胞生物学会发育生物学分会副会长等, 并获得谈家桢生命科学创新奖, 钟南山青年科技奖等

摘要：

In mammals, miRNAs recognize target mRNAs via base pairing, which leads to a complex ‘multiple-to-multiple' regulatory network. Previous studies have focused on the regulatory mechanisms and functions of individual miRNAs, but alterations of many individual miRNAs do not strongly disturb the miRNA regulatory network. Our recent studies revealed the important roles of global miRNA dosage control in stem cell fate decisions, which can be further used to optimize liver organoid culturing to obtain mature liver cells. Particularly we used plant immune protein RDR to rescue miRNA dosage deficiency specifically in cancer cells, suggesting that methods of controlling global miRNA dosage may serve as effective therapeutic tools to cure human diseases.

主讲人发表论文摘选：

1. Cui, Y., Qi, Y., Ding, L., Ding, S., Han, Z., Wang, Y., Du, P., 2024. miRNA dosage control in development and human disease. Trends in Cell Biology 34, 31–47.

2. Du, P., Wu, J., 2024. Hallmarks of totipotent and pluripotent stem cell states. Cell Stem Cell 31, 312–333

3. Li, S., Yang, M., Shen, H., Ding, L., Lyu, X., Lin, K., Ong, J., Du, P., 2024. Capturing totipotency in human cells through spliceosomal repression. Cell 187, 3284-3302.e23

4. Lyu, X., Cui, Y., Kong, Y., Yang, M., Shen, H., Liao, S., Li, S., An, C., Wang, H., Zhang, Z., Ong, J., Li, Y., Du, P., 2024. A transient transcriptional activation governs unpolarized-to-polarized morphogenesis during embryo implantation. Molecular Cell 84, 2665-2681.e13

5. Li, Y., Li, Z., Wang, Changliang, Yang, M., He, Z., Wang, F., Zhang, Y., Li, R., Gong, Y., Wang, B., Fan, B., Wang, Chunyue, Chen, L., Li, H., Shi, P., Wang, N., Wei, Z., Wang, Y.-L., Jin, L., Du, P., Dong, J., Jiao, J., 2023. Spatiotemporal transcriptome atlas reveals the regional specification of the developing human brain. Cell 186, 5892-5909.e22. https://doi.org/10.1016/j.cell.2023.11.016

6. Yang, M., Ong, J., Meng, F., Zhang, F., Shen, H., Kitt, K., Liu, T., Tao, W., Du, P., 2023. Spatiotemporal insight into early pregnancy governed by immune-featured stromal cells. Cell 186, 4271-4288.e24

7. Li, Y., Li, Z., Yang, M., Wang, F., Zhang, Y., Li, R., Li, Q., Gong, Y., Wang, B., Fan, B., Wang, C., Chen, L., Li, H., Ong, J., Teng, Z., Jin, L., Wang, Y.-L., Du, P., Jiao, J., 2022. Decoding the temporal and regional specification of microglia in the developing human brain. Cell Stem Cell 29, 620-634.e6

8. Qi, Y., Ding, L., Zhang, S., Yao, S., Ong, J., Li, Y., Wu, H., Du, P., 2022b. A plant immune protein enables broad antitumor response by rescuing microRNA deficiency. Cell 185, 1888-1904.e24