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孙前文

 

孙前文 博士

研究员,博士生导师,国家自然科学基金委 优秀青年基金 (2018)

2014-今        助理教授,清华大学生命科学学院

2014-今        研究员,清华-北大生命科学联合中心

2009-2014    博士后,John Innes Centre, Norwich, UK

2004-2009    博士  华中农业大学生科院

2000-2004    学士 华中农业大学生科院

本实验室研究方向

  我们实验室感兴趣各个层面的基因组调控事件,重点关注R-loop生物学研究。R-loop是由一条单链DNA和另一条RNA:DNA杂合链混合组成的三股染色质结构,在许多生物体中发挥着重要的生物功能。我们以拟南芥和其它模式生物(如大肠杆菌和酵母)为模型来研究R-loop生物学的基本问题,如R-loop形成、稳定与解除的分子机理,以及R-loop如何影响转录调控和基因组维持。

 

已发表论文:

l 2015年至今 (*corresponding or co-corresponding authors; #co-first authors)

20. Yan P#, Zunpeng Liu#, Song M#, Wu Z, Xu W, Li K, Ji Q, Wang S, Liu X, Yan K, Esteban R, Ci W, Xie W, Ren J, Belmonte J, Zhang W*, Sun Q*, Qu J*, Liu GH*. Genome-wide R-loop landscapes during cell differentiation and reprogramming. Cell Reports, 2020 Jul 7; 32(1):107870.

19. Li Y#, Song Y#, Xu W#, Li Q#, Wang X, Li K, Wang J, Liu Z, Velychko S, Ye R, Xia Q, Wang L, Guo R, Dong X, Zheng Z, Dai Y, Li H, Yao M, Xue Y, Schöler H, Sun Q*, Yao H*. R-loops Coordinate with SOX2 in Regulating Reprogramming to Pluripotency. Science Advances, 2020 Jun; 6(24), eaba0777.

18. Xu W#, Li K#, Li S#, Hou Q#, Zhang Y, Liu K and Sun Q*. The R-loop atlas of Arabidopsis development and responses to environmental stimuli. The Plant Cell. 2020 Apr; 32(4):888-903.

17. Yang Z, Li M, Sun Q*. RHON1 Co-transcriptionally Resolves R-Loops for Arabidopsis Chloroplast Genome Maintenance. Cell Reports. 2020 Jan; 30(1), 243–256.

16. Zhou J#, Liu L#, Li Q, Xu W, Li K, Wang ZW, Sun Q*. Intronic heterochromatin prevents cryptic transcription initiation in Arabidopsis. The Plant Journal. 2020 Mar;101(5):1185-1197.

15. Yang X#, Liu QL#, Xu W#, Zhang YC#, Yang Y, Ju LF, Chen J, Chen YS, Li K, Ren J*, Sun Q*, Yang YG*. m6A promotes R-loop formation to facilitate transcription termination. Cell Research. 2019 Dec; 29(12):1035-1038.

14. Yuan W#, Zhou J#, Tong J#, Zhuo W, Wang L, Li Y, Sun Q*, Qian W*, ALBA protein complex reads genic R-loops to maintain genome stability in Arabidopsis. Science Advances, 2019 May 15;5(5): eaav9040.

13. Zhao S#, Cheng L#, Gao Y#, Zhang B#, Zheng X, Wang L, Li P*, Sun Q*, Li H*. Plant HP1 protein ADCP1 links multivalent H3K9 methylation readout to heterochromatin formation. Cell Research. 2019 Jan;29(1):54-66.

12. Cheng L, Shafiq S, Xu W, Sun Q*. EARLY FLOWERING IN SHORT DAYS (EFS) regulates the seed size in Arabidopsis. Sci China Life Sci. 2018 Feb;61(2):214-224.

11. Yang Z#, Hou Q#, Cheng L#, Xu W, Hong Y, Li S, Sun Q*. RNase H1 Cooperates with DNA Gyrases to Restrict R-Loops and Maintain Genome Integrity in Arabidopsis Chloroplasts. The Plant Cell. 2017 Oct;29(10):2478-2497.

10. Xu W, Xu H, Li K, Fan Y, Liu Y, Yang X*, Sun Q*. The R-loop is a common chromatin feature of the Arabidopsis genome. Nature Plants. 2017 Sep;3(9):704-714.

9. Shafiq S#, Chen C#*, Yang J, Cheng L, Ma F, Widemann E, Sun Q*. DNA Topoisomerase 1 Prevents R-loop Accumulation to Modulate Auxin-Regulated Root Development in Rice. Molecular Plant. 2017 Jun;10(6):821-833.

8. Shafiq S, Li J, Sun Q*. Functions of plants long non-coding RNAs. Biochim Biophys Acta. 2016 Jan;1859(1):155-62.

 

l 2015年之前:

7. Csorba T#, Questa JI#, Sun Q# and Dean C. Antisense COOLAIR mediates the coordinated switching of chromatin states at FLC during vernalization. Proc Natl Acad Sci USA. 2014. 111:16160-5 (#contributed equally).

6. Wang ZW, Wu Z, Raitskin O, Sun Q and Dean C. Antisense-mediated FLC transcriptional repression requires the P-TEFb transcription elongation factor. Proc Natl Acad Sci USA. 2014. 111:7468-73.

5. Marquardt S, Raitskin O, Wu Z, Liu F, Sun Q and Dean C. Functional Consequences of Splicing of the Antisense Transcript COOLAIR on FLC Transcription. Molecular Cell. 2014. 54:156-65.

4. Sun Q, Csorba T, Skourti-Stathaki K, Proudfoot NJ and Dean C. R-Loop Stabilization Represses Antisense Transcription at the Arabidopsis FLC Locus. Science. 2013. 340:619-21.

3. Qin FJ, Sun QW, Huang LM, Chen XS and Zhou DX. Rice SUVH histone methyltransferase genes display specific functions in chromatin modification and retrotransposon repression. Molecular Plant. 2010. 3:773-82.

2. Sun Q and Zhou DX. Rice jmjC domain-containing gene JMJ706 encodes H3K9 demethylase required for floral organ development. Proc Natl Acad Sci USA. 2008. 105:13679-84.

1. Huang L#, Sun Q#, Qin F, Li C, Zhao Y and Zhou DX. Down-regulation of a SILENT INFORMATION REGULATOR2-related histone deacetylase gene, OsSRT1, induces DNA fragmentation and cell death in rice. Plant Physiol. 2007. 144:1508-19. (#contributed equally).

 

联系方式:

地址:清华大学生物技术馆4段313室(办公室),309室(实验室)

电话: +86-10-62784002 (办公室); 62796360 (实验室)

 

电子邮件:sunqianwen(a t)mail.tsinghua.edu.cn

 

 

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