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Sai Li


Sai Li 




Research Area

My lab focuses on cryo-electron tomography (cryo-ET) and its applications in in situ structural biology. We aim at solving sub-nanometer resolution structure of proteins from their functional states in native contexts. We are active in developing cryo-ET methods, including: 1) cryo-ET and high resolution subtomogram averaging, 2) Focused-ion beam milling of samples (FIB-SEM), and 3) Correlative light and electron microscopy (CLEM) in cryo conditions. These methods are primarily applied on studying 1) native structures of protein, 2) ultrastructure of macro-complexes, including interactions between protein, nuclear acids, membrane and 4) enveloped virus-host cell interactions.


Recent Publications

1.        Halldorsson S*, Li S*, Li M, Harlos K, Bowden TA, Huiskonen JT. Shielding and activation of a viral membrane fusion protein. Nat Commun. 2018; 9: 349. doi: 10.1038/s41467-017-02789-2.

2.        Watanabe Y, Raghwani J, Allen JD, Seabright GE, Li S, Moser F, Huiskonen JT, Strecker T, Bowden TA, Crispin M. Structure of the Lassa virus glycan shield provides a model for immunological resistance. Proceedings of the National Academy of Sciences, 2018, 115(28): 7320.

3.        Li S, Rissanen I, Zeltina A, Hepojoki J, Raghwani J, Harlos K, Pybus OG, Huiskonen JT, Bowden TA. A molecular-level account of the antigenic hantaviral surface. Cell Rep. 2016;16(1):278. doi: 10.1016/j.celrep.2016.06.039.

4.        Li S, Sun Z, Pryce R, Parsy ML, Fehling SK, Schlie K, Siebert CA, Garten W, Bowden TA, Strecker T, Huiskonen JT.Acidic pH-induced conformations and LAMP1 binding of the Lassa virus glycoprotein spike. PLoS Pathog. 2016; 12(2):e1005418. doi: 10.1371/journal.ppat.1005418.

5.        Huiskonen JT, Parsy ML, Li S, Bitto D, Renner M, Bowden TA. Averaging of viral envelope glycoprotein spikes from electron cryotomography reconstructions using Jsubtomo. J Vis Exp. 2014 Oct 21;(92):e51714. doi: 10.3791/51714.

6.        Li S*, Sieben C*, Ludwig K, Höfer CT, Chiantia S, Herrmann A, Eghiaian F, Schaap IA. pH-Controlled two-step uncoating of influenza virus. Biophys J. 2014; 106(7):1447-56. doi: 10.1016/j.bpj.2014.02.018.

7.        Li S, Eghiaian F, Sieben C, Herrmann A, Schaap IA. Bending and puncturing the influenza lipid envelope.Biophys J. 2011; 100(3): 637–645. doi: 10.1016/ j.bpj.2010.12.3701.

8.        Hoos F, Li S, Meyrath TP, Braun B, Giessen H. Thermal lensing in an end-pumped Yb: KGW slab laser with high power single emitter diodes. Opt Express. 2008 Apr 28; 16(9):6041-9.



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