吴嘉炜

 

研究方向 :
1. 糖尿病、肥胖症等疾病中关键蛋白的结构和功能研究
2. TGF-信号通路的负调控机制
3. Wnt信号转导中骨架蛋白Axin及其相互作用蛋白的研究
4. MAPK信号通路中激酶和磷酸酶活力调控的分子机制

 

代表性论文：

1. Wang L, Liu YT, Hao R, Chen L, Chang Z, Wang HR, Wang ZX, Wu JW*. (2011) Molecular mechanism of the negative regulation of Smad1/5 protein by carboxyl terminus of Hsc70-interacting protein (CHIP). J Biol Chem. 286(18):15883-94.

2. Liu YT, Dan QJ, Wang J, Feng Y, Chen L, Liang J, Li Q, Lin SC, Wang ZX, Wu JW*. (2011) Molecular basis of Wnt activation via the DIX-domain protein Ccd1. J Biol Chem. 286(10):8597-608.

3. Zhu L, Chen L, Zhou XM, Zhang YY, Zhang YJ, Zhao J, Ji SR, Wu JW*, Wu Y*. (2011) Structural insights into the architecture and allostery of full-length AMP-activated protein kinase. Structure. 19(4):515-22.

4. Wang F, Li H, Wang L, Yang WC, Wu JW*, Yang GF*. (2011) Design, syntheses, and kinetic evaluation of 3-(phenylamino)oxazolidine-2,4-diones as potent cytochrome bc1 complex inhibitors. Bioorg Med Chem. 19(15):4608-15.

5. Gao X, Wang J, Yu DQ, Bian F, Xie BB, Chen XL, Zhou BC, Lai LH, Wang ZX, Wu JW*, Zhang YZ*. (2010) Structural basis for the autoprocessing of zinc metalloproteases in the thermolysin family. Proc Natl Acad Sci U S A. 107(41):17569-74.

6. Zhao PL, Wang L, Zhu XL, Huang X, Zhan CG*, Wu JW*, Yang GF*. (2010) Subnanomolar Inhibitor of Cytochrome bc(1) Complex Designed by Optimizing Interaction with Conformationally Flexible Residues. J Am Chem Soc. 132(1):185-94.

7. Chen L, Jiao ZH, Zheng LS, Zhang YY, Xie ST, Wang ZX, Wu JW*. (2009) Structural insight into the autoinhibition mechanism of AMP-activated protein kinase. Nature. 459(7250):1146-9.

8. Zhang YY, Mei ZQ, Wu JW*, Wang ZX*. (2008) Enzymatic activity and substrate specificity of mitogen-activated protein kinase p38alpha in different phosphorylation states. J Biol Chem. 283(39):26591-601.

9. Wang ZX*, Wu JW.* (2007) The complete pathway for ERK2-catalyzed reaction. Evidence for an iso random Bi-Bi mechanism. J Biol Chem. 282(38):27678-84.

10. Yan N, Wu JW, Chai J, Li W, Shi Y. (2004) Molecular mechanisms of DrICE inhibition by DIAP1 and removal of inhibition by Reaper, Hid and Grim. Nat Struct Mol Biol. 11(5):420-8.

11. Chai J, Wu JW, Yan N, Massague J, Pavletich NP, Shi Y. (2003) Features of a Smad3 MH1-DNA complex. Roles of water and zinc in DNA binding. J Biol Chem. 278(22):20327-31.

12. Wu JW, Krawitz AR, Chai J, Li W, Luo K, Shi Y. (2002) Structural mechanism of Smad4 recognition by the nuclear oncoprotein Ski: insights on Ski-mediated repression of TGF-beta signaling. Cell. 111(3):357-367.

13. Wu JW, Hu M, Chai J, Seoane J, Huse M, Li C, Rigotti DJ, Kyin S, Muir TW, Fairman R, Massague J, Shi Y. (2001) Crystal structure of a phosphorylated Smad2. Recognition of phosphoserine by the MH2 domain and on Smad function in TGF-beta signaling. Mol Cell. 8(6):1277-89. (Cover story)

14. Wu JW, Cocina AE, Chai J, Hay BA, Shi Y. (2001) Structural analysis of a functional DIAP1 fragment bound to grim and hid peptides. Mol Cell. 8(1):95-104.

15. Wu JW, Fairman R, Penry J, Shi Y. (2001) Formation of a stable heterodimer between Smad2 and Smad4. J Biol Chem. 276(23):20688-94.

16. Wu JW, Wu Y, Wang ZX. (2001) Kinetic analysis of a simplified scheme of autocatalytic zymogen activation. Eur. J. Biochem. 268(6):1547-53.

17. Wu JW, Wang ZX. (1999) New Evidence for the Denaturant Binding Model, Protein Sci. 8(10):2090-2097.

18. Wu JW, Wang ZX. (1998) Activation Mechanism and Modification Kinetics of Chinese Hamster Dihydrofolate Reductase by p-Chloromercuribenzoate, Biochem. J. 335(1):181-189.

19. Wu JW, Wang ZX, Zhou JM. (1997) Three-State Kinetic Analysis of Chinese Hamster Dihydrofolate Reductase Unfolding by Guanidine Hydrochloride, Biochim. Biophys. Acta 1343(1):107-116.

20. Wu JW, Wang ZX, Zhou JM. (1997) Inactivation Kinetics of Dihydrofolate Reductase from Chinese Hamster during Urea Denaturation, Biochem. J. 324(2):395-401.