生命科学联合中心

谢晓亮

谢晓亮

电子邮件： sunneyxie@pku.edu.cn

实验室电话：010-62752373

研究方向

谢晓亮教授是美国科学院院士、美国医学院院士、中科院外籍院士。他是单分子酶学的创始人、单分子生物物理化学的奠基人之一、相干拉曼散射显微成像技术和单细胞基因组学的开拓者。2012年谢晓亮组开发了单细胞全基因组均匀扩增的新方法—多重退火循环扩增法（MALBAC）。目前该技术已帮助数百个中国患有单基因遗传病的家庭成功避免了疾病的子代传递。谢晓亮教授课题组的研究方向包含三个领域：

（1）基础研究：单分子酶学、单分子生物物理化学、基因表达和调控、表观遗传学、细胞分化和重编程机理、基因组不稳定性;

（2）技术开发：单分子成像、单细胞基因组学、相干拉曼散射显微成像、DNA测序;

（3）医学转化：体外受精胚胎植入前遗传学筛查与诊断、早期癌症诊断。

代表性科研论文 / Selected Publications：

1.Chen C, Xing D, Tan L, Li H, Zhou G, Huang L, Xie X *(2017) "Single-cell whole-genome analyses by Linear Amplification via Transposon Insertion (LIANTI)," Science 356, 189-194.

2.Zhou Y, Wang P, Tian F, Gao G, Huang L, Wei W, Xie X *(2017)"Painting a specific chromosome with CRISPR/Cas9 for live-cell imaging," Cell Res 27, 298-301.

3. Fu D, Yang W, Xie X *(2017) "Label-free Imaging of Neurotransmitter Acetylcholine at Neuromuscular Junctions with Stimulated Raman Scattering," J Am Chem Soc 139, 583−586.

4.Yan L, Huang L, Xu L, Huang J, Ma F, Zhu X, Tang Y, Liu M, Lian Y, Liu P, Li R, Lu S, Tang F, Qiao J, Xie X *(2015) "Live births after simultaneous avoidance of monogenic diseases and chromosome abnormality by next-generation sequencing with linkage analyses," ProcNatlAcadSci USA 112, 15964-15969.

5. Chong S, Chen C, Ge H, Xie X* (2014) "Mechanism of Transcriptional Bursting in Bacteria," Cell 158, 314-326.

6. Hou Y, Fan W, Yan L, Li R, Lian Y, Huang J, Li J, Xu L, Tang F, Xie X *, Qiao J (2013)"Genome analyses of single human oocytes," Cell 155, 1492-1506.

7.Kim S, Brostromer E, Xing D, Jin J, Chong S, Ge H, Wang S, Gu C, Yang L, Gao Y, Su X, Sun Y, Xie X* (2013) "Probing Allostery Through DNA," Science 339, 816-819.

8.Zong C, Lu S, Chapman A, Xie X*(2012) "Genome-wide detection of single-nucleotide and copy-number variations of a single human cell," Science 338, 1622-1626.