学术报告
题 目: Bacterial Protein Translocation: a single molecule study
报告人: Prof. Arnold J.M. Driessen
Department of Molecular Microbiology, Groningen Biomolecular Science and Biotechnology Institute, and the Zernike Institute for Advanced Materials, University of Groningen, The Netherlands
时 间: 2012年11月27日下午3:00
地 点: 化学楼A205
Abstrarct:
The heterotrimeric SecYEG complex comprises a protein-conducting channel in the bacterial cytoplasmic membrane. SecYEG functions together with the motor protein SecA in preprotein translocation. SecYEG can also associate with a translating ribosome to mediate membrane protein insertion. We have used single molecule techniques, advanced membrane protein reconstitution methods and chemical biology approaches to address mechanistic and structural questions on the protein translocase.
References:
Bonardi, F., London, G., Nouwen, N., Feringa, B.L., Driessen, A.J.M. (2010) Light induced control of protein translocation via the SecYEG complex. Angewandte Chemie Int. Ed. 49: 7234-7238.
Kusters, I., van den Bogaart, G., Kedrov, A., Krasnikov, V., Fulyani, F., Poolman, B., Driessen, A.J.M. (2011) Dynamics of SecA oligomerization in solution and bound to SecYEG Probed at the single molecule level. Structure 19:430-439.
Bonardi, F., Halza, E., Walko, M., Du Plessis, F., Nouwen, N., Feringa, B.L., Driessen, A.J.M. (2011) Probing the SecYEG translocation pore aize with preproteins conjugated with sizable rigid spherical molecules. Proc. Natl. Acad. Sci. USA 108: 7775-7780.
Kedrov, A., Kusters, I., Krasnikov, V.V., Driessen, A.J.M. (2011) A single copy of SecYEG is sufficient for preprotein translocation. EMBO J. 30: 4387-4397
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