生命科学联合中心
学术报告
Title：Applying synthetic chemistry to address long standing challenges at the interface of chemistry and biology: Chemically Modified DNAzymes, 18F-Labelling Methodology, First Total Synthesis Amanitin.
Speaker： David M. Perrin, Ph.D.
Professor, Department of Chemistry
The University of British Columbia
Time：2018-5-10（周四），13:00 -14:00 pm
Venue：北京大学化学与分子工程学院A717报告厅
Host：刘志博，北大-清华生命科学联合中心
I am so honored to be invited by my former Chinese students to China. My lab has worked on long-standing challenges in bioorganic chemistry. Three challenges that we have worked on for approximately a decade include: 1) Sequence specific ribonuclease-A mimics; 2) an efficient means of 18F-labeling biomolecules for use in PET imaging and 3) the totally synthesis of alphaamanitin. For the first aim, we have developed modified deoxynucleoside triphosphates that present functionalities commonly found in proteins to deliver DNAzyme activity against specific RNA targets in a manner that mimics RNaseA. For the second aim, we have exploited the unique chemistry of boron to deliver radioactive 18F-fluoride, in a single step, to bioconjugates appended with a boronic acid. Finally, for the third aim, we have appreciated the considerable and enduring interest in alpha-amanitin, one of the deadliest peptide toxins known, for applications in chemical biology and for use in antibody-drug conjugates. For the past 60 years, there has been no synthetic source of this toxin. In seeking to empower the development of antibody drug conjugates, we have recently reported the first total synthesis of this historic toxin. Endeavors in these three challenges will be highlighted.
