Modern Analytical Ultracentrifugation Sedimentation Velocity in the Study of Protein Interactions
现代分析超高速分析离心沉降速率技术和其在蛋白质相互作用研究中的应用
Peter Schuck，Ph.D.
Principal Investigator, Dynamics of Macromolecular Assembly Section, LCIMB/NIBIB,
National Institutes of Health, Bethesda, U.S.A.
Biography：
Dr. Schuck obtained his Ph.D. from the Goethe-University Frankfurt in Main, Germany, where he worked on interactions of integral proteins of the erythrocyte membrane using analytical ultracentrifugation. He received his post-doctoral research training in physical biochemistry with Dr. Allen Minton at NIDDK. In 2014 he was appointed an Earl Stadtman Investigator in NIBIB and Chief of the Dynamics of Macromolecular Assembly Section in the Laboratory of Cellular Imaging and Macromolecular Biophysics.
Abstract：
The dynamic assembly of multi-protein complexes underlies fundamental processes in cell biology. A mechanistic understanding of the assembly of macromolecular species, which frequently form multiple co-existing complexes, requires accurate measurement their stoichiometry, affinity and cooperativity. Sedimentation velocity analytical ultracentrifugation is a traditional biophysical technique that allows the characterization of interacting proteins and their complexes free in solution by monitoring changes in the migration patterns caused by transient binding events. Most recently, we have developed tools for fluorescence detection sedimentation velocity (FDS-SV) and demonstrated that it can be used to study protein interactions even in the low picomolar concentration range. Further, we extended the capabilities of FDS-SV with a single excitation wavelength from single-component to multi-component detection using photoswitchable fluorescent proteins (psFPs). Due to the combination of interaction analysis with the hydrodynamic size-resolution, the range of applications for FDS-SV far surpasses most other popular methods for studying high-affinity interactions, such as isothermal titration calorimetry and surface plasmon resonance biosensing, and fluorescence anisotropy.
Time: Sep. 29st, 2017, 15:30
Venue: New Biology Building, Room 143
Host: Prof. Hongwei Wang
举办单位：生命科学联合中心
